山羊F(ab')2抗兔IgG Fc (Alkaline Phosphatase) (ab6024)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG Fc (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC
Related conjugates and formulations
概述
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产品名称
山羊F(ab')2抗兔IgG Fc (Alkaline Phosphatase)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Rabbit IgG F(ab) .
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经测试应用
适用于: ICC/IF, Dot blot, WB, ELISA, IHCmore details -
免疫原
Rabbit IgG F(ab')2 fragment
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偶联物
Alkaline Phosphatase
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
F(ab')2 Anti-Rabbit IgG F(c) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | ||
| Dot blot | ||
| WB | ||
| ELISA | ||
| IHC |
| 说明 |
|---|
应用说明
Dot: Use at an assay dependent dilution.
ELISA: 1/600 - 1/2800.
IHC: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/600 to 1/2800 of the reconstitution concentration is suggested for this product.
Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/600 - 1/2800.
IHC: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/600 to 1/2800 of the reconstitution concentration is suggested for this product.
Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab6024 尚未被引用在任何文献中。