山羊F(ab')2抗兔IgG F(ab')2 (HRP)预吸附二抗(ab6112)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, Electron Microscopy, IHC
概述
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产品名称
山羊F(ab')2抗兔IgG F(ab')2 (HRP)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.. -
经测试应用
适用于: ICC/IF, IHC-P, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
最小交叉反应
Cow, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Rabbit IgG F(ab’)2 fragment
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
应用 | Ab评论 | 说明 |
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ICC/IF |
1/1000 - 1/5000.
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IHC-P |
1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Dot blot |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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ELISA |
1/100000.
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
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Electron Microscopy |
Use at an assay dependent concentration.
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IHC |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
1/1000 - 1/5000. |
IHC-P
1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Dot blot
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ELISA
1/100000. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
Electron Microscopy
Use at an assay dependent concentration. |
IHC
Use at an assay dependent concentration. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6112, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (43)
ab6112 被引用在 43 文献中.
- Zhang J et al. Comparison of the effect of rotenone and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine on inducing chronic Parkinson's disease in mouse models. Mol Med Rep 25:N/A (2022). PubMed: 35039876
- Li H et al. Gene therapy of yeast NDI1 on mitochondrial complex I dysfunction in rotenone-induced Parkinson's disease models in vitro and vivo. Mol Med 28:29 (2022). PubMed: 35255803
- Wang Y et al. Overexpression of ARHI increases the sensitivity of cervical cancer cells to paclitaxel through inducing apoptosis and autophagy. Drug Dev Res 83:142-149 (2022). PubMed: 34189759
- Li S et al. CD155/TIGIT signaling regulates the effector function of tumor-infiltrating CD8+ T cell by NF-κB pathway in colorectal cancer. J Gastroenterol Hepatol 37:154-163 (2022). PubMed: 34734434
- Liu Y et al. Circular RNA ROCK1, a novel circRNA, suppresses osteosarcoma proliferation and migration via altering the miR-532-5p/PTEN axis. Exp Mol Med 54:1024-1037 (2022). PubMed: 35879346