山羊F(ab')2抗兔IgG - (Fab')2 (DyLight® 650),预吸附二抗(ab102294)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG - (Fab')2 (DyLight® 650), pre-adsorbed
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC, Flow Cyt
Related conjugates and formulations
概述
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产品名称
山羊F(ab')2抗兔IgG - (Fab')2 (DyLight® 650),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species. -
经测试应用
适用于: IHC-P, ICC, Flow Cytmore details -
最小交叉反应
Human, Mouse, Rat more details -
偶联物
DyLight® 650. Ex: 654nm, Em: 673nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 650. -
克隆
多克隆 -
同种型
IgG -
常规说明
DyLight® 650 replaces DyLight® 649. -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab102294于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50 - 1/500.
|
|
ICC |
1/50 - 1/500.
|
|
Flow Cyt |
1/50 - 1/200.
|
说明 |
---|
IHC-P
1/50 - 1/500. |
ICC
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab102294 被引用在 1 文献中.
- Liu C et al. Dopamine neurons in the ventral periaqueductal gray modulate isoflurane anesthesia in rats. CNS Neurosci Ther 26:1121-1133 (2020). PubMed: 32881314