山羊F(ab')2抗小鼠IgM mu chain (TRITC) (ab5928)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (TRITC)
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Functional Studies, ICC/IF, Immunomicroscopy, Flow Cyt
概述
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产品名称
山羊F(ab')2抗小鼠IgM mu chain (TRITC)
参阅全部 IgM 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. -
经测试应用
适用于: Functional Studies, ICC/IF, Immunomicroscopy, Flow Cytmore details -
免疫原
Mouse IgM mu chain
-
偶联物
TRITC. Ex: 547nm, Em: 572nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG F(ab’)2 -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
1/10000 - 1/50000.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| Flow Cyt |
1/500 - 1/2500.
|
| 说明 |
|---|
|
Functional Studies
1/10000 - 1/50000. |
|
ICC/IF
1/1000 - 1/5000. |
|
Immunomicroscopy
Use at an assay dependent dilution. |
|
Flow Cyt
1/500 - 1/2500. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab5928 被引用在 2 文献中.
- Bataille A et al. In Vitro Differentiation of Human Skin-Derived Cells into Functional Sensory Neurons-Like. Cells 9:N/A (2020). PubMed: 32316463
- Ishikawa M et al. Neurosteroids are endogenous neuroprotectants in an ex vivo glaucoma model. Invest Ophthalmol Vis Sci 55:8531-41 (2014). ICC/IF ; Rat . PubMed: 25406290
