山羊F(ab')2抗小鼠IgM mu chain (HRP) (ab5930)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-Fr, Dot blot, WB, ELISA, Electron Microscopy, IHC
概述
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产品名称
山羊F(ab')2抗小鼠IgM mu chain (HRP)
参阅全部 IgM 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. -
经测试应用
适用于: ICC/IF, IHC-Fr, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
免疫原
Mouse IgM mu chain
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偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.87% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading...
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纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/500 - 1/5000.
|
|
IHC-Fr |
Use at an assay dependent dilution.
|
|
Dot blot |
1/5000 - 1/100000.
|
|
WB |
1/2000 - 1/10000.
|
|
ELISA |
1/90000.
|
|
Electron Microscopy |
Use at an assay dependent dilution.
|
|
IHC |
1/500 - 1/5000.
|
说明 |
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ICC/IF
1/500 - 1/5000. |
IHC-Fr
Use at an assay dependent dilution. |
Dot blot
1/5000 - 1/100000. |
WB
1/2000 - 1/10000. |
ELISA
1/90000. |
Electron Microscopy
Use at an assay dependent dilution. |
IHC
1/500 - 1/5000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab5930 被引用在 12 文献中.
- Nagase H et al. Reliable and sensitive detection of glycosaminoglycan chains with immunoblots. Glycobiology 31:116-125 (2021). PubMed: 32614944
- Casagrande FVM et al. The imbalance between dynamic and stable microtubules underlies neurodegeneration induced by 2,5-hexanedione. Biochim Biophys Acta Mol Basis Dis 1866:165581 (2020). PubMed: 31672549
- Hou X et al. Wnt signaling regulates cytosolic translocation of connexin 43. Am J Physiol Regul Integr Comp Physiol 317:R248-R261 (2019). PubMed: 31067079
- Lynn MA et al. Early-Life Antibiotic-Driven Dysbiosis Leads to Dysregulated Vaccine Immune Responses in Mice. Cell Host Microbe 23:653-660.e5 (2018). PubMed: 29746836
- Xu W et al. Emission of sulfur dioxide from polyurethane foam and respiratory health effects. Environ Pollut 242:90-97 (2018). PubMed: 29960929