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山羊F(ab')2抗小鼠IgM mu chain (FITC) (ab5926)

Submit a review Q&A (3)References (2)
Flow Cytometry - Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)

    Key features and details

    • Goat F(ab')2 Anti-Mouse IgM mu chain (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC/IF, Flow Cyt, ELISA

    概述

    • 产品名称

      山羊F(ab')2抗小鼠IgM mu chain (FITC)
      参阅全部 IgM 二抗

    • 宿主

      Goat

    • 靶标种属

      Mouse

    • 特异性

      Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.

    • 经测试应用

      适用于: ICC/IF, Flow Cyt, ELISAmore details

    • 免疫原

      Mouse IgM mu chain

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
    • Concentration information loading...

    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

    • 共轭说明

      Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of Goat IgG F(ab’)2

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab5926于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    1/1000 - 1/5000.
    Flow Cyt
    1/500 - 1/2500.
    ELISA
    1/10000 - 1/50000.
    说明
    ICC/IF
    1/1000 - 1/5000.
    Flow Cyt
    1/500 - 1/2500.
    ELISA
    1/10000 - 1/50000.

    图片

    • Flow Cytometry - Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)
      Flow Cytometry - Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)

      ab5926 was used at dilution 1/100 with the primary antibody ab2784 in Flow Cyt. See the review on ab2784.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (2)

    发表研究结果有使用 ab5926？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab5926 被引用在 2 文献中.

    • Zhong Y  et al. Spheres derived from the human SK-RC-42 renal cell carcinoma cell line are enriched in cancer stem cells. Cancer Lett 299:150-60 (2010). Flow Cyt . PubMed: 20846785
    • Wang K  et al. Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells. Cloning Stem Cells 11:483-96 (2009). ICC/IF ; Rhesus monkey, Human . PubMed: 20025521

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Question

    human blood cells confirmed by PCR to be B35 positive. secondary rat IGM supposed to react with mouse. primary 1/10 to neat. FACS

    Read More
    Answer

    Hello and thank you for your patience is trying to troublshooting your Flow Cytometry experiment. I have noticed the secondary you are using is specific to the mu chain of mouse IgM. The primary antibodies are to total IgM, so this is likely the reason you are only getting a portion of the staining. I would suggest using a secondary like ab47830.

    Read More

    Question

    Dear Sir/Madam,

    I am writing for technical support. Recently, we have purchased a vial of mouse anti-connexin43 antibody (ab11369) from Abcam. We didn't notice that it is IgM isotype, thus we did not get positive signals from our first trial of this antibody. We are now considering purchase a vial of goat anti-mouse IgM antibody (ab5926). We are wondering whether this one reacts with other normally used secondary antibodies, such as goat anti-rabbit IgG and goat anti-mouse IgG, as we are going to do some co-stainings.

    Read More
    Answer

    Thank you for contacting us.

    ab5926 shows less than 1% cross reactivity against other mouse heavy or light chain isotypes in ELISA.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Does this cross-react with human IgM ? I am looking for a Fab-fragment secondary antibody to human IgM for use in flow cytometry and possibly also confocal microscopy.

    Read More
    Answer

    Thank you for your enquiry. Ab5926 has not been tested for cross-reactivity with human IgM, just mouse IgM. We do have several human IgM secondary antibodies (for example ab8506), but unfortunately not a Fab-fragment secondary antibody to human IgM. To see if another company may have what you are looking for, you can click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogues of all online antibody companies (currently 249). If you have any additional questions, please contact us again.

    Read More

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