山羊F(ab')2抗小鼠IgG+IgM+IgA H&L (FITC)预吸附二抗(ab5999)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (FITC) preadsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, ELISA
概述
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产品名称
山羊F(ab')2抗小鼠IgG+IgM+IgA H&L (FITC)预吸附二抗
参阅全部 IgG + IgM + IgA 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins. -
经测试应用
适用于: Flow Cyt, ICC/IF, ELISAmore details -
最小交叉反应
Human more details -
免疫原
Mouse IgG, IgA, and IgM whole molecules
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偶联物
FITC. Ex: 493nm, Em: 528nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 4.0 moles FITC per mole of Goat IgG F(ab’)2 -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/500 - 1/2500.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| ELISA |
1/10000 - 1/50000.
|
| 说明 |
|---|
|
Flow Cyt
1/500 - 1/2500. |
|
ICC/IF
1/1000 - 1/5000. |
|
ELISA
1/10000 - 1/50000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (4)
ab5999 被引用在 4 文献中.
- Khaled SAA et al. Quantitative Assay of Mutated Nucleophosmin in Acute Myeloid Leukemia. J Hematol 8:111-120 (2019). PubMed: 32300454
- Hutchinson CV et al. Lymphocytes from chronic lymphocytic leukaemia undergo ABL1-linked amoeboid motility and homotypic interaction as an early adaptive change to ex vivo culture. Exp Hematol Oncol 3:7 (2014). PubMed: 24618035
- Mosiolek M et al. Rumex acetosa Y chromosomes: constitutive or facultative heterochromatin? Folia Histochem Cytobiol 43:161-7 (2005). PubMed: 16201317
- Xia B et al. Versatile fluorescent derivatization of glycans for glycomic analysis. Nat Methods 2:845-50 (2005). PubMed: 16278655
