山羊F(ab')2抗小鼠IgG+IgM+IgA - H&L (Biotin),预吸附二抗(ab6005)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG+IgM+IgA - H&L (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy
概述
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产品名称
山羊F(ab')2抗小鼠IgG+IgM+IgA - H&L (Biotin),预吸附二抗
参阅全部 IgG + IgM + IgA 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum. No reaction was observed against Human Serum proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations. -
经测试应用
适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details -
最小交叉反应
Human more details -
免疫原
Mouse IgG, IgA and IgM whole molecules
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偶联物
Biotin
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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纯化说明
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/2000 - 1/20000.
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|
Dot blot |
Use at an assay dependent dilution.
|
|
WB |
1/5000 - 1/20000.
|
|
ELISA |
1/1.2e+006.
|
|
IHC |
Use at an assay dependent dilution.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
说明 |
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ICC/IF
1/2000 - 1/20000. |
Dot blot
Use at an assay dependent dilution. |
WB
1/5000 - 1/20000. |
ELISA
1/1.2e+006. |
IHC
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab6005 被引用在 1 文献中.
- Pahwa S et al. Characterization and regulation of MT1-MMP cell surface-associated activity. Chem Biol Drug Des N/A:N/A (2018). PubMed: 30480376