山羊F(ab')2抗小鼠IgG - Fc (PE),预吸附二抗(ab5881)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG - Fc (PE), pre-adsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt
概述
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产品名称
山羊F(ab')2抗小鼠IgG - Fc (PE),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab) or Bovine, Horse or Human Serum Proteins.
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经测试应用
适用于: ICC/IF, Immunomicroscopy, Flow Cytmore details -
最小交叉反应
Cow, Horse, Human more details -
免疫原
Mouse IgG F(c) fragment
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偶联物
PE. Ex: 488nm, Em: 575nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
BSA is IgG and protease free -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound) -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
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| Immunomicroscopy |
Use at an assay dependent dilution.
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| Flow Cyt |
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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| 说明 |
|---|
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ICC/IF
1/100 - 1/250. |
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Immunomicroscopy
Use at an assay dependent dilution. |
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Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab5881 被引用在 4 文献中.
- Manganelli V et al. Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs. Autophagy N/A:1-21 (2020). PubMed: 33034545
- Huang S et al. Cellular communication promotes mammosphere growth and collective invasion through microtubule-like structures and angiogenesis. Oncol Rep 40:3297-3312 (2018). PubMed: 30542711
- Tao J et al. Association between B7-H1 and cervical cancer: B7-H1 impairs the immune response in human cervical cancer cells. Exp Ther Med 14:4125-4133 (2017). PubMed: 29104629
- Tjiam MC et al. Viremic HIV Controllers Exhibit High Plasmacytoid Dendritic Cell-Reactive Opsonophagocytic IgG Antibody Responses against HIV-1 p24 Associated with Greater Antibody Isotype Diversification. J Immunol 194:5320-8 (2015). PubMed: 25911748