山羊F(ab')2抗小鼠IgG - Fc (HRP),预吸附二抗(ab5879)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, WB, Dot blot, ELISA, Electron Microscopy, IHC
概述
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产品名称
山羊F(ab')2抗小鼠IgG - Fc (HRP),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse, Human Serum Proteins. -
经测试应用
适用于: ICC/IF, WB, Dot blot, ELISA, Electron Microscopy, IHCmore details -
最小交叉反应
Cow, Horse, Human more details -
免疫原
Mouse IgG F(c) whole molecule
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偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/250 - 1/1000.
|
|
| WB |
1/500 - 1/2000.
|
|
| Dot blot |
Use at an assay dependent dilution.
|
|
| ELISA |
1/2000.
|
|
| Electron Microscopy |
Use at an assay dependent dilution.
|
|
| IHC |
Use at an assay dependent dilution.
|
| 说明 |
|---|
|
ICC/IF
1/250 - 1/1000. |
|
WB
1/500 - 1/2000. |
|
Dot blot
Use at an assay dependent dilution. |
|
ELISA
1/2000. |
|
Electron Microscopy
Use at an assay dependent dilution. |
|
IHC
Use at an assay dependent dilution. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (7)
ab5879 被引用在 7 文献中.
- Ou S et al. S100A16 suppresses the proliferation, migration and invasion of colorectal cancer cells in part via the JNK/p38 MAPK pathway. Mol Med Rep 23:N/A (2021). PubMed: 33355370
- Liu H et al. Dendritic cell-derived exosomal miR-494-3p promotes angiogenesis following myocardial infarction. Int J Mol Med 47:315-325 (2021). PubMed: 33416108
- Li Y et al. Overexpression of G3BP1 facilitates the progression of colon cancer by activating ß-catenin signaling. Mol Med Rep 22:4403-4411 (2020). PubMed: 33000280
- Pu J et al. Isolated double adrenocorticotropic hormone-secreting pituitary adenomas: A case report and review of the literature. Oncol Lett 12:585-590 (2016). IHC-P ; Human . PubMed: 27347184
- Li H et al. Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA). Sci Rep 5:14918 (2015). PubMed: 26446566
- Yoshikawa T et al. Dynamic innate immune responses of human bronchial epithelial cells to severe acute respiratory syndrome-associated coronavirus infection. PLoS One 5:e8729 (2010). WB . PubMed: 20090954
- Froeling FE et al. Organotypic culture model of pancreatic cancer demonstrates that stromal cells modulate E-cadherin, beta-catenin, and Ezrin expression in tumor cells. Am J Pathol 175:636-48 (2009). PubMed: 19608876
