山羊F(ab')2抗小鼠IgG - Fc (AP),预吸附二抗(ab5880)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG - Fc (AP), pre-adsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC
概述
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产品名称
山羊F(ab')2抗小鼠IgG - Fc (AP),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab)Â or Bovine, Horse and Human Serum Proteins.
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经测试应用
适用于: ICC/IF, Dot blot, WB, ELISA, IHCmore details -
最小交叉反应
Cow, Horse, Human more details -
免疫原
Mouse IgG F(c) fragment
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偶联物
Alkaline Phosphatase
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
BSA is IgG and protease free -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/200 - 1/1000.
|
|
| Dot blot |
Use at an assay dependent dilution.
|
|
| WB |
1/500 - 1/2500.
|
|
| ELISA |
1/2000 - 1/10000.
|
|
| IHC |
Use at an assay dependent dilution.
|
| 说明 |
|---|
|
ICC/IF
1/200 - 1/1000. |
|
Dot blot
Use at an assay dependent dilution. |
|
WB
1/500 - 1/2500. |
|
ELISA
1/2000 - 1/10000. |
|
IHC
Use at an assay dependent dilution. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab5880 被引用在 2 文献中.
- Ono Y et al. Scleraxis-lineage cells are required for correct muscle patterning. Development 150:N/A (2023). PubMed: 37246520
- Maguire RJ et al. Early transcriptional targets of MyoD link myogenesis and somitogenesis. Dev Biol 371:256-68 (2012). PubMed: 22954963