山羊F(ab')2抗小鼠IgG - (Fab)'2 (TRITC ),预吸附二抗(ab5885)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (TRITC ), pre-adsorbed
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA
Related conjugates and formulations
概述
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产品名称
山羊F(ab')2抗小鼠IgG - (Fab)'2 (TRITC ),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. -
经测试应用
适用于: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details -
最小交叉反应
Cow, Horse, Human, Rabbit, Rat, Sheep more details -
免疫原
Mouse IgG F(ab)2 fragment
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偶联物
TRITC. Ex: 547nm, Em: 572nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.4 moles TRITC per mole of Goat IgG F(ab’)2 -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| Flow Cyt |
1/500 - 1/2500.
|
|
| ELISA |
1/10000 - 1/50000.
|
| 说明 |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
Immunomicroscopy
Use at an assay dependent dilution. |
|
Flow Cyt
1/500 - 1/2500. |
|
ELISA
1/10000 - 1/50000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab5885 被引用在 2 文献中.
- Donis N et al. Differential Biological Effects of Dietary Lipids and Irradiation on the Aorta, Aortic Valve, and the Mitral Valve. Front Cardiovasc Med 9:839720 (2022). PubMed: 35295264
- Bencsik P et al. Moderate inhibition of myocardial matrix metalloproteinase-2 by ilomastat is cardioprotective. Pharmacol Res 80:36-42 (2014). ICC/IF . PubMed: 24380772
