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山羊F(ab')2抗小鼠IgG - (Fab')2 (Biotin),预吸附二抗(ab5886)

Submit a review Q&A (1)References (3)

Key features and details

  • Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy

Conjugates logo Related conjugates and formulations

DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC HRP HRP PE TRITC Unconjugated

概述

  • 产品名称

    山羊F(ab')2抗小鼠IgG - (Fab')2 (Biotin),预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Mouse

  • 特异性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgGF(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

  • 经测试应用

    适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details

  • 最小交叉反应


    Cow, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Mouse IgG F(ab)2 fragment

  • 偶联物

    Biotin

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 共轭说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5886于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/2000 - 1/10000.
ELISA
1/20000 - 1/100000.
IHC
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
说明
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/2000 - 1/10000.
ELISA
1/20000 - 1/100000.
IHC
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (3)

发表研究结果有使用 ab5886？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab5886 被引用在 3 文献中.

  • Di Persio S  et al. Single-cell RNA-seq unravels alterations of the human spermatogonial stem cell compartment in patients with impaired spermatogenesis. Cell Rep Med 2:100395 (2021). PubMed: 34622232
  • Onishi Y  et al. Relationship between lamellar sensory corpuscles distributed along the upper arm's deep arteries and pulsating sensation of blood vessels. J Anat 239:101-110 (2021). PubMed: 33527396
  • Schneider F  et al. Serum and intratesticular inhibin B, AMH, and spermatogonial numbers in trans women at gender-confirming surgery: An observational study. Andrology 9:1781-1789 (2021). PubMed: 34085780

客户评价及客户问答

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Question

Hi,



I'm looking for a biotin labelled F(ab')2 antimouse antibody for peroxidase immunohistochemistry (lung tissue --> first antibody = monoclonal antibody IgG1 that detects adducts of hypoxyprobe). ABCAM provides three different antibodies; ab98657, ab98756 and ab5886. How do these products differ from each other? And what does pre-adsorbed means?



Many thanks in advance for your help!

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Answer

Thank you for your enquiry and your interest in our products.
Although, all these three secondary antibodies (ab5886, ab98756, ab98657) recognize mouse IgG and were raised in goat, they are from different sources. The tested application/species/concentration may vary.
Pre-adsorbed antibodies (ab5886, ab98756) are prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. This means that they only recognize mouse IgG and any cross-reactivity with other species is minimal.
I hope this helps and if I can assist further, please do not hesitate to contact me.

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