山羊F(ab')2抗人IgG H&L (PE)预吸附二抗(ab7006)
Key features and details
- Goat F(ab')2 Anti-Human IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Dot blot, ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt
概述
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产品名称
山羊F(ab')2抗人IgG H&L (PE)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Human -
特异性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), or Bovine, Horse, Mouse and Rat Serum Proteins.
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经测试应用
适用于: Dot blot, ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details -
最小交叉反应
Cow, Horse, Mouse, Rat more details -
免疫原
Full length native Human IgG (purified).
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偶联物
PE. Ex: 488nm, Em: 575nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 Phycobiliprotein Concentration: 0.5 mg / ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound). -
克隆
多克隆 -
同种型
IgG -
常规说明
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
Use at an assay dependent concentration.
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| ICC/IF |
1/100 - 1/250.
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| IHC-P |
1/50 - 1/200.
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| IHC-Fr |
1/50 - 1/200.
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| Immunomicroscopy |
1/50 - 1/200.
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| Flow Cyt |
1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
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| 说明 |
|---|
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Dot blot
Use at an assay dependent concentration. |
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ICC/IF
1/100 - 1/250. |
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IHC-P
1/50 - 1/200. |
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IHC-Fr
1/50 - 1/200. |
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Immunomicroscopy
1/50 - 1/200. |
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Flow Cyt
1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab7006 被引用在 6 文献中.
- Asashima H et al. Prior cycles of anti-CD20 antibodies affect antibody responses after repeated SARS-CoV-2 mRNA vaccination. JCI Insight 8:N/A (2023). PubMed: 37606046
- Alexandru N et al. Intravenous Administration of Allogenic Cell-Derived Microvesicles of Healthy Origins Defend Against Atherosclerotic Cardiovascular Disease Development by a Direct Action on Endothelial Progenitor Cells. Cells 9:N/A (2020). PubMed: 32059493
- Tiwari A et al. Controlling the Effective Oxygen Tension Experienced by Cells Using a Dynamic Culture Technique for Hematopoietic Ex Vivo Expansion. Curr Protoc Stem Cell Biol 44:2A.11.1-2A.11.13 (2018). PubMed: 29512129
- Tiwari A et al. Expansion of Human Hematopoietic Stem/Progenitor Cells on Decellularized Matrix Scaffolds. Curr Protoc Stem Cell Biol 36:1C.15.1-1C.15.16 (2016). PubMed: 26840223
- Alvarez-Cienfuegos A et al. Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains. Sci Rep 6:28643 (2016). PubMed: 27345490
- Tiwari A et al. Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds. Curr Protoc Stem Cell Biol 28:Unit 1C.15. (2014). PubMed: 24510768