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Immunology Immunoglobulins Heavy Chain IgG

山羊F(ab)抗大鼠IgG H&L (Biotin) (ab7176)

  • Datasheet
Submit a review Q&A (1)References (2)

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Key features and details

  • Goat F(ab) Anti-Rat IgG H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy

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概述

  • 产品名称

    山羊F(ab)抗大鼠IgG H&L (Biotin)
    参阅全部 IgG 二抗
  • 宿主

    Goat
  • 靶标种属

    Rat
  • 特异性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
  • 经测试应用

    适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • 免疫原

    Rat IgG whole molecule

  • 偶联物

    Biotin

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 6.50
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    IgG and protease free
  • Concentration information loading...
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • 共轭说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG Fab molecule
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rat
    • IgG
    • Biotin

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7176于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Dot blot
WB
ELISA
IHC
Immunomicroscopy
说明
应用说明
Dot: Use at an assay dependent dilution.
ELISA: 1/20000 - 1/110000.
IHC: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.

This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20000 to 1/110000 of the reconstitution concentration is suggested for this product.

Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (2)

发表研究结果有使用 ab7176?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7176 被引用在 2 文献中.

  • Liu H  et al. Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis. Oxid Med Cell Longev 2022:8011850 (2022). PubMed: 35419170
  • Yang Y  et al. Cryptotanshinone alleviates polycystic ovary syndrome in rats by regulating the HMGB1/TLR4/NF-?B signaling pathway. Mol Med Rep 22:3851-3861 (2020). PubMed: 32901834

客户评价及客户问答

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Question

I need a anti-rat biotin conjugated secondary Ab raised in goat to perform IHC on human joint tissue. In addition to ab6275, you have ab6619, 6256, 7176 in your catalog that match that description. Can you tell me if there is any difference between these antibodies and which one you recommend for this application (IHC)?

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Abcam community

Verified customer

Asked on Jan 04 2007

Answer

Thank you for your enquiry. Ab6275: Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(ab')2 [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is raised against Rat F(ab')2. Ab7176: Biotin Conjugated Fab Fragment of Affinity Purified anti-Rat IgG [H&L] [Goat] - This product is the Fab portion (single Fab fragments) of the Goat IgG raised against Rat IgG. Ab6256:Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(c) [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is raised against Rat F(c). Typically Fab and Fab2 fragments will bind less than the entire IgG molecule. This means these types of products are good for assays in which very low background is desired. I hope I was able to make things more clear. Please do not hesitate to contact me if you have any other questions regarding this issue.

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Abcam Scientific Support

回复于 Jan 08 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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