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山羊F(ab)抗兔IgG H&L (HRP) (ab7171)

Submit a review Q&A (1)References (14)

Key features and details

  • Goat F(ab) Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC

Conjugates logo Related conjugates and formulations

Biotin FITC Texas Red ® TRITC Unconjugated

概述

  • 产品名称

    山羊F(ab)抗兔IgG H&L (HRP)
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Rabbit

  • 特异性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).

  • 经测试应用

    适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details

  • 免疫原

    Rabbit IgG whole molecule

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    IgG and protease free
  • Concentration information loading...

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

  • 共轭说明

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7171于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/1000 - 1/5000.
ELISA
1/10000 - 1/50000.
Electron Microscopy
Use at an assay dependent dilution.
IHC
Use at an assay dependent dilution.
说明
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/1000 - 1/5000.
ELISA
1/10000 - 1/50000.
Electron Microscopy
Use at an assay dependent dilution.
IHC
Use at an assay dependent dilution.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (14)

发表研究结果有使用 ab7171？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7171 被引用在 14 文献中.

  • Fan R  et al. Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats. Evid Based Complement Alternat Med 2022:2112359 (2022). PubMed: 35432561
  • Shaw IW  et al. A role for steroid 5 alpha-reductase 1 in vascular remodeling during endometrial decidualization. Front Endocrinol (Lausanne) 13:1027164 (2022). PubMed: 36465608
  • Nikiforova N  et al. CM-Dil Staining and SEC of Plasma as an Approach to Increase Sensitivity of Extracellular Nanovesicles Quantification by Bead-Assisted Flow Cytometry. Membranes (Basel) 11:N/A (2021). PubMed: 34357175
  • Guan Y  et al. Protective multi-target effects of DL-3-n-butylphthalide combined with 3-methyl-1-phenyl-2-pyrazolin-5-one in mice with ischemic stroke. Mol Med Rep 24:N/A (2021). PubMed: 34643246
  • Sakamoto Y  et al. High-Fat Diet and Age-Dependent Effects of IgA-Bearing Cell Populations in the Small Intestinal Lamina Propria in Mice. Int J Mol Sci 22:N/A (2021). PubMed: 33503874
View all Publications for this product

客户评价及客户问答

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Question

How about if this antibody ab8888 (anti-Dopamine) conduct for mouse brain sample IHC experiment?
Besides, customer consider use monoclonal antibody such as ab8892(anti-Dopamine) use for mouse sample IHC.
Customer concern cross reaction of secondary anibody.
Which secondary antibody you suggest since their brain sample also from mouse.
Thanks a lot for your assistance.
Best regards,

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Answer

Thank you for contacting us.

If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse species to avoid cross reactivity.

We have some secondaries in our catalogue raised in Goat that could be appropriate for your assay:

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-ab-2-F-ab-2-Fragment-ab6112.html

https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Biotin-pre-adsorbed-ab6012.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-c-F-ab-2-Fragment-ab6021.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-F-ab-2-Fragment-ab6013.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Fab-Fragment-ab7171.html

It is also very important to use a blocking agent such as serum from the same species as the secondary was raised in. If you used any of the secondaries suggested, therefore, 10% goat serum would be the most suitable blocking agent.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

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