山羊F(ab)抗小鼠IgG H&L (FITC) (ab6669)
Key features and details
- Goat F(ab) Anti-Mouse IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISA
Related conjugates and formulations
概述
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产品名称
山羊F(ab)抗小鼠IgG H&L (FITC)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). -
经测试应用
适用于: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details -
免疫原
Mouse IgG whole molecule
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偶联物
FITC. Ex: 493nm, Em: 528nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 5.0 moles FITC per mole of Goat IgG Fab -
克隆
多克隆 -
同种型
IgG -
常规说明
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| Flow Cyt | (1) |
Use at an assay dependent dilution.
|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| ELISA |
1/10000 - 1/50000.
|
| 说明 |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
Flow Cyt
Use at an assay dependent dilution. |
|
Immunomicroscopy
Use at an assay dependent dilution. |
|
ELISA
1/10000 - 1/50000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (4)
ab6669 被引用在 4 文献中.
- Ollington B et al. Immunoresponsive Tissue-Engineered Oral Mucosal Equivalents Containing Macrophages. Tissue Eng Part C Methods 27:462-471 (2021). PubMed: 34210153
- Kochegarov A et al. A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos. J Biomed Sci 20:20 (2013). PubMed: 23522091
- Mogilenko DA et al. Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages. FASEB J : (2012). PubMed: 22271762
- Mogilenko DA et al. Modified low density lipoprotein stimulates complement C3 expression and secretion via liver X receptor and toll-like receptor 4 activation in human macrophages. J Biol Chem : (2011). PubMed: 22194611
