山羊F(ab)抗小鼠IgG H&L (Biotin) (ab6822)
Key features and details
- Goat F(ab) Anti-Mouse IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: Dot blot, WB, ELISA, IHC, Immunomicroscopy, ICC/IF
Related conjugates and formulations
概述
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产品名称
山羊F(ab)抗小鼠IgG H&L (Biotin)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c). -
经测试应用
适用于: Dot blot, WB, ELISA, IHC, Immunomicroscopy, ICC/IFmore details -
免疫原
Mouse IgG whole molecule
-
偶联物
Biotin
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. -
共轭说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG Fab molecule -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
Use at an assay dependent dilution.
|
|
| WB |
1/2000 - 1/10000.
|
|
| ELISA |
1/200000.
|
|
| IHC |
Use at an assay dependent dilution.
|
|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
| 说明 |
|---|
|
Dot blot
Use at an assay dependent dilution. |
|
WB
1/2000 - 1/10000. |
|
ELISA
1/200000. |
|
IHC
Use at an assay dependent dilution. |
|
Immunomicroscopy
Use at an assay dependent dilution. |
|
ICC/IF
1/1000 - 1/5000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (2)
ab6822 被引用在 2 文献中.
- Jiang F et al. Fluorofenidone attenuates paraquat-induced pulmonary fibrosis by regulating the PI3K/Akt/mTOR signaling pathway and autophagy. Mol Med Rep 23:N/A (2021). PubMed: 33786626
- O'Connor RA et al. T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer. Oncoimmunology 10:1940675 (2021). PubMed: 34290905
