山羊F(ab)抗小鼠IgG H&L (ab6668)
Key features and details
- Goat F(ab) Anti-Mouse IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ICC/IF, IHC, ELISA
Related conjugates and formulations
概述
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产品名称
山羊F(ab)抗小鼠IgG H&L
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: WB, ICC/IF, IHC, ELISAmore details -
免疫原
Mouse IgG whole molecule
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).” -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000.
|
|
ICC/IF |
1/1000 - 1/5000.
|
|
IHC |
1/1000 - 1/5000.
|
|
ELISA |
1/50000.
|
说明 |
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WB
1/2000 - 1/10000. |
ICC/IF
1/1000 - 1/5000. |
IHC
1/1000 - 1/5000. |
ELISA
1/50000. |
图片
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SDS-PAGE results of Goat Fab Anti-Mouse IgG (H&L) ab6668.
Lane 1: Non-reduced Goat Fab Anti-Mouse.
Lane 2: Opal Prestained Ladder.
Lane 3: Reduced Goat Fab Anti-Mouse.
Load: 1.0 µg.
Coomassie stained and imaged.
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Dot Blot results of Goat Fab Anti-Mouse IgG Antibody ab6668.
Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng.
Blocked for 30 min at RT.
Primary Antibody: Goat Fab Anti-Mouse IgG at 1 µg/mL for 1 hr at RT.
Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40,000 for 30 min at RT.
Imaged with BioRad ChemiDoc, Chemi filter.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (29)
ab6668 被引用在 29 文献中.
- Kokotović T et al. Transcription factor mesenchyme homeobox protein 2 (MEOX2) modulates nociceptor function. FEBS J 289:3457-3476 (2022). PubMed: 35029322
- Xue Y et al. Mechanical tension mobilizes Lgr6+ epidermal stem cells to drive skin growth. Sci Adv 8:eabl8698 (2022). PubMed: 35476447
- van Loon K et al. Cancer Vaccination against Extracellular Vimentin Efficiently Adjuvanted with Montanide ISA 720/CpG. Cancers (Basel) 14:N/A (2022). PubMed: 35681575
- Mey GM et al. Visual imaging as a predictor of neurodegeneration in experimental autoimmune demyelination and multiple sclerosis. Acta Neuropathol Commun 10:87 (2022). PubMed: 35706005
- Azimova D et al. Cbp1, a fungal virulence factor under positive selection, forms an effector complex that drives macrophage lysis. PLoS Pathog 18:e1010417 (2022). PubMed: 35731824