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山羊抗鸡IgY H&L预吸附二抗(ab7113)

Submit a review Q&A (1)References (1)

Key features and details

  • Goat Anti-Chicken IgY H&L preadsorbed
  • Host species: Goat
  • Suitable for: WB, ICC/IF, IHC-Fr, IHC-P, IP, Immunodiffusion, Conjugation, ELISA

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概述

  • 产品名称

    山羊抗鸡IgY H&L预吸附二抗
    参阅全部 IgY 二抗

  • 宿主

    Goat

  • 靶标种属

    Chicken

  • 特异性

    No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.

  • 经测试应用

    适用于: WB, ICC/IF, IHC-Fr, IHC-P, IP, Immunodiffusion, Conjugation, ELISAmore details

  • 最小交叉反应


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native Chicken IgY (purified).

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer.

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7113于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/2000 - 1/10000.
ICC/IF
1/1000 - 1/5000.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ELISA
1/600000.
说明
WB
1/2000 - 1/10000.
ICC/IF
1/1000 - 1/5000.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
ELISA
1/600000.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab7113？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7113 被引用在 1 文献中.

  • Almada P  et al. Automating multimodal microscopy with NanoJ-Fluidics. Nat Commun 10:1223 (2019). PubMed: 30874553

客户评价及客户问答

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Question

Nice talked with you on the phone about the our order 1773Ab. We are so sorry about the results and send the results to you (see attach file). The protocol of Dot blot are following: 3 ul of serum and TK1puri. were dot on the NC membrane and then blocked in TBST with 10 % non-fat milk for 4 hours . The presence or absence of the primary (anti-thymidine kinase1,TK1) antibody (we produced) was incubated at +4 C over night After incubation with a biotinylated secondary antibody (from Abcom 7113 6000x dilution or from Jackson 10000x dilution) was added for one hour at room temperature. The membrane was then incubated in TBS buffer with avidin-HRP-streptavidin (from Amersham). Finaly ECl detection was reaction 1 min and then exposed to X-ray film. The intensity of a single spot on the film was determined by a Laser Densitometer. From the intensity of the rhTK1 . Results: Now we found the higher cross-reaction between Abcom 7113 and serum sample, did not find for the second Ab from Jackson. See fig. We really hepe that you will give an answer soon.. And We aslo found the 7113 Ab without 50% glycerol for stored at -20 C. We are sorry about te results.

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Answer

Thank you for contacting us for technical support and for sending us all this protocol information and an image, it is very useful to understand your problem. The antibody ab7113 has not been tested for DotBlot so we do not know if it will work in this application and we cannot guarantee it will. I think the problem you are having may be due to the blocking: I would recommend trying 5% milk or 5%BSA in TBST to block, only for 1hour, then add the primary and then the secondary antibodies (diluted in TBST only, no milk). This will help remove the non specific binding. I hope this suggestion will help you, please let me know if I can be of further assistance,

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