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山羊抗鸡IgY H&L (Biotin)预吸附二抗(ab7117)

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Key features and details

  • Goat Anti-Chicken IgY H&L (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    山羊抗鸡IgY H&L (Biotin)预吸附二抗
    参阅全部 IgY 二抗

  • 宿主

    Goat

  • 靶标种属

    Chicken

  • 特异性

    No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.

  • 经测试应用

    适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • 最小交叉反应


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native chicken IgY (purified).

  • 偶联物

    Biotin

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    Anti-Chicken IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer.

  • 共轭说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7117于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
说明
应用说明
Dot: Use at an assay dependant dilution.
ELISA: 1/8000 - 1/36000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab7117？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7117 尚未被引用在任何文献中。

客户评价及客户问答

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Question

Thanks for your help, but I am still uncertain about my question regarding the addition of glycerol. "The storage instructions stated to add 50% glycerol before freezing. Should this be 50% of the current volume or a final concentration of 50%".

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Answer

Thank you for your message. I have double-checked with my colleagues and actually we have reconstituted this antibody and already added 50% glycerol to the buffer. This means that for long term storage, you do not need to add anything just aliquot the product and keep it at -20°C. We have corrected our on-line datasheet in order to avoid more confusion. Thank you again for bringing this matter to our attention.

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