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山羊抗鸡IgY H&L (Alkaline Phosphatase)预吸附二抗(ab7119)

Submit a review Q&A (1)

Key features and details

  • Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed
  • Conjugation: Alkaline Phosphatase
  • Host species: Goat
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

概述

  • 产品名称

    山羊抗鸡IgY H&L (Alkaline Phosphatase)预吸附二抗
    参阅全部 IgY 二抗

  • 宿主

    Goat

  • 靶标种属

    Chicken

  • 特异性

    No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.

  • 经测试应用

    适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • 最小交叉反应


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native chicken IgY (purified).

  • 偶联物

    Alkaline Phosphatase

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer.

  • 共轭说明

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7119于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
说明
应用说明
Dot: Use at an assay dependant dilution.
ELISA: 1/1000 - 1/4000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab7119？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7119 尚未被引用在任何文献中。

客户评价及客户问答

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Question

Could you tell me the difference between ab7119 and ab6878 in immunoblotting examination?　My target molecules are from ruminants including bovine.　From your data sheet I should use ab7119 rather than ab6878 due to lower background.　Please give me your suggestion.

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Answer

Thank you for your inquiry. I can confirm that both recognize whole chicken IgY and IgG (H+L).　However, ab7119 has been cross adsorbed to remove reactivity with bovine,　goat, guinea pig, hamster, horse, human, mouse, rabbit, rat and sheep serum　proteins. Meanwhile, ab6878 has not been cross adsorbed. Therefore, I would suggest using ab7119 instead as it will have less cross reactivity with bovine. I hope this information helps. If there is anything else that I can help you with, please do not hesitate to contact me.

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