山羊抗鸡IgY H&L (Alexa Fluor® 568)预吸附二抗

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 568) preadsorbed (AB175711)

ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 10μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175711 Alexa Fluor® 568 goat anti-chicken IgY (H+L) preadsorbed used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® 568 is pseudocolored in yellow.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 568) preadsorbed (AB175711)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175711 Alexa Fluor® 568 goat anti-chicken IgY (H+L) preadsorbed used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® 568 is pseudocolored in yellow.

• Alexa Fluor®

Supplier Data

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 568) preadsorbed (AB175711)

Emission spectra of Alexa Fluor^® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.