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山羊抗亚美尼亚仓鼠IgG H&L (Texas Red ®) (ab5743)

Submit a review Q&A (3)References (1)

Key features and details

  • Goat Anti-Armenian hamster IgG H&L (Texas Red ®)
  • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA, WB

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin FITC TRITC Unconjugated

概述

  • 产品名称

    山羊抗亚美尼亚仓鼠IgG H&L (Texas Red ®)
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Armenian hamster

  • 经测试应用

    适用于: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA, WBmore details

  • 免疫原

    Armenian Hamster IgG whole molecule.

  • 偶联物

    Texas Red ®. Ex: 596nm, Em: 620nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 6.50
    Preservative: 0.01% Sodium azide
    Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA

    BSA is protease free
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.1 moles Texas Red per mole of Goat IgG

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5743于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/1000 - 1/5000.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
1/500 - 1/2500.
ELISA
1/10000 - 1/50000.
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
说明
ICC/IF
1/1000 - 1/5000.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
1/500 - 1/2500.
ELISA
1/10000 - 1/50000.
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab5743？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab5743 被引用在 1 文献中.

  • Su N  et al. Occurrence of Transmembrane Protein 119 in the Retina is Not Restricted to the Microglia: An Immunohistochemical Study. Transl Vis Sci Technol 8:29 (2019). PubMed: 31853425

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Yes, it is correct. ************does the purchasing in this group usually. I am a visiting professor here.

Feel free to contact him to double-check, he is the one who gave me the customer ID.

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Answer

Thank you for your reply.

I have sent the quote for the products requested to the email address we have on file for **********.

When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

If there is anything else I can help you with, please let me know.

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Question

Concerning the quote, the customer number is**********

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Answer

Thank you for your reply.

Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

Thank you for your patience, and I look forward to your reply.

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Question

I selected the following antibodies from the ABcam catalog:

Primary:

Anti-CD63 antibody [AD1] (FITC)

ab108949

$337.00



Anti-CD62P antibody [Psel.KO.2.7]

ab54427

$337.00



Anti-CD42d antibody [1C2]

ab95780

$337.00



Secondary:


Goat anti-Mouse IgG H&L (Cy5.5 ®)

ab6947

$185.00



Goat anti-Mouse IgG1 heavy chain (FITC)

ab97239

$165.00



Goat polyclonal to Armenian Hamster (TR)

ab5743

$165.00



Isotype controls:


MOUSE IgG1 antibody [MOPC-21] (FITC)

ab106163

$325.00



Mouse IgG1 [ICIGG1] - Isotype Control

ab91353

$315.00



Armenian Hamster IgG monoclonal [HTK888] - Isotype control

ab18479

$325.00



Two questions:

For the secondary antibody, will the first one (ab6947) work against ab54427, or do we need to buy the second one (ab97239)? One is against IgG, the other one is against IgG1. ab54427 is IgG1.

If you could double-check the selection for compatibility (e.g., correct isotype controls) and then ask the sales department to offer us a quote, that would be great.

Read More
Answer

Thank you for your reply.

The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please send me your current email address and I will generate the quote.

I look forward to your reply.

Read More

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