山羊抗亚美尼亚仓鼠IgG H&L (Biotin) (ab5744)
Key features and details
- Goat Anti-Armenian hamster IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ICC/IF, Dot blot, ELISA, Immunomicroscopy, IHC-P
Related conjugates and formulations
概述
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产品名称
山羊抗亚美尼亚仓鼠IgG H&L (Biotin)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Armenian hamster -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum. Cross reactivity against mouse has not been determined. -
经测试应用
适用于: WB, ICC/IF, Dot blot, ELISA, Immunomicroscopy, IHC-Pmore details -
免疫原
Armenian Hamster IgG whole molecule
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偶联物
Biotin
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000.
|
|
ICC/IF |
1/1000 - 1/5000.
|
|
Dot blot |
Use at an assay dependent dilution.
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|
ELISA |
1/250000.
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|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
IHC-P |
Use at an assay dependent dilution.
|
说明 |
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WB
1/2000 - 1/10000. |
ICC/IF
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
ELISA
1/250000. |
Immunomicroscopy
Use at an assay dependent dilution. |
IHC-P
Use at an assay dependent dilution. |
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab5744 被引用在 9 文献中.
- Zheng J et al. Evaluation of metastatic niches in distant organs after surgical removal of tumor-bearing lymph nodes. BMC Cancer 18:608 (2018). PubMed: 29848296
- Dinh CH et al. Bardoxolone Methyl Prevents High-Fat Diet-Induced Colon Inflammation in Mice. J Histochem Cytochem 64:237-55 (2016). PubMed: 26920068
- Wang S et al. Chronic rhein treatment improves recognition memory in high-fat diet-induced obese male mice. J Nutr Biochem 36:42-50 (2016). PubMed: 27567591
- Onodera K et al. GATA2 regulates dendritic cell differentiation. Blood 128:508-18 (2016). PubMed: 27259979
- Dinh CH et al. Bardoxolone Methyl Prevents Mesenteric Fat Deposition and Inflammation in High-Fat Diet Mice. ScientificWorldJournal 2015:549352 (2015). IHC-P . PubMed: 26618193
- Dinh CH et al. Bardoxolone Methyl Prevents Fat Deposition and Inflammation in Brown Adipose Tissue and Enhances Sympathetic Activity in Mice Fed a High-Fat Diet. Nutrients 7:4705-23 (2015). IHC-P . PubMed: 26066016
- Accart N et al. Revisiting fixation and embedding techniques for optimal detection of dendritic cell subsets in tissues. J Histochem Cytochem 62:661-71 (2014). PubMed: 24874853
- Yu P et al. Diet-induced obesity drives negative mouse vein graft wall remodeling. J Vasc Surg N/A:N/A (2013). PubMed: 23876511
- Dahllöf MS et al. The lysine deacetylase inhibitor Givinostat inhibits ß-cell IL-1ß induced IL-1ß transcription and processing. Islets 4:417-22 (0). PubMed: 23486342