为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Immunology Immunoglobulins Heavy Chain IgG

驴抗羊IgG H&L (FITC) (ab6896)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (5)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Key features and details

  • Donkey Anti-Sheep IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Donkey
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 650 Gold 12nm HRP Texas Red ® TRITC Unconjugated

你可能会感兴趣

Western blot
Product image
InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
偶联
Product image
Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)
封闭
Product image
Normal Donkey Serum (ab7475)

查看更多相关产品

概述

  • 产品名称

    驴抗羊IgG H&L (FITC)
    参阅全部 IgG 二抗
  • 宿主

    Donkey
  • 靶标种属

    Sheep
  • 经测试应用

    适用于: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details
  • 免疫原

    Full length native Sheep whole (purified).

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • 克隆

    多克隆
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Sheep
    • IgG
    • Fluorophore
    • FITC

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6896于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
IHC-P
IHC-Fr (1)
Immunomicroscopy
Flow Cyt
ELISA
说明
应用说明
Flow Cyt: Use at an assay dependant concentration.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (5)

发表研究结果有使用 ab6896?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6896 被引用在 5 文献中.

  • Cui H  et al. Protocol to image and quantify nucleocytoplasmic transport in cultured cells using fluorescent in situ hybridization and a dual reporter system. STAR Protoc 3:101813 (2022). PubMed: 36386872
  • Luo RY  et al. ProBDNF promotes sepsis-associated encephalopathy in mice by dampening the immune activity of meningeal CD4+ T cells. J Neuroinflammation 17:169 (2020). PubMed: 32466783
  • Allen TA  et al. Circulating tumor cells exit circulation while maintaining multicellularity, augmenting metastatic potential. J Cell Sci 132:N/A (2019). PubMed: 31409692
  • Lee CC  et al. Naa10p Inhibits Beige Adipocyte-Mediated Thermogenesis through N-a-acetylation of Pgc1a. Mol Cell 76:500-515.e8 (2019). PubMed: 31422874
  • Holm LJ  et al. Fenofibrate increases very-long-chain sphingolipids and improves blood glucose homeostasis in NOD mice. Diabetologia N/A:N/A (2019). PubMed: 31410530

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-4 of 4 Abreviews or Q&A

Detection of sheep anti tyrosine hydroxylase labelling of splenic nerves

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
I used this antibody to detect sheep anti tyrosine hydroxylase (TH) labelling of splenic nerves in mouse spleen sections along with rat anti-CD35 labelling of follicular dendritic cells (FDC) and rabbit anti PrP labelling of prion protein. It was diluted 1/200 for use on frozen sections that were fixed in acetone and blocked with serum for 30 mins at room temperature. I chose this antibody as I found it difficult to find secondary's that were anti-sheep. I was concerned that the FITC might bleach during confocal microscopy however I was able to scan the triple labelling without any bleaching occurring.

Image is of red CD35+ FDC, blue PrP and green TH+ nerves detected using donkey anti-sheep_FITC
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Laura McCulloch

Verified customer

提交于 Oct 23 2013

Question

We have already ordered antibodies from other company. I just asked my boss. You can refund our money on our account. Nice to contact with you and thank you very much for your help!

Read More

Abcam community

Verified customer

Asked on Nov 09 2011

Answer

I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

Read More

Abcam Scientific Support

回复于 Nov 09 2011

Question

Yes, the image of DAKO gfap staining is the typical astrocyte, just as the image of instruction your company provided to us with ab90601 antibody. So, you can see the significant differences!

Read More

Abcam community

Verified customer

Asked on Nov 08 2011

Answer

Thanks for your reply. I am very sorry these anti-GFAP and anti-MAP2 antibodies continue to give you trouble. I'd be happy to send you any two replacement antibodies from our catalog. Or if you prefer I can set up a credit or refund instead. Please let me know how you would like to proceed.

Read More

Abcam Scientific Support

回复于 Nov 08 2011

Question

to whom may be concerned: our lab ordered four antibodies from your company in July. we cannot find the order list now. ab90601 sheep polyconal to GFAP. ab11267 mouse monoclonal HM-2 to MAP2 - neuronal marker and other two secondary antibodies: ab6896 Donkey polyclonal secondary antibody to sheep IgG - H&L(FITC) and ab47052 Goat polyclonal secondary antibody to Mouse IgG - H&L(AMCA). we used the GFAP to stain astrocyte of rat brain frozen sections and MAP2 to stain neuron of rat brain frozen sections. However, we found the images of GFAP staining were very similar to neurons, clearly not astrocytes. we repeat experiment at least three times and obtain the same results. last time, we borrowed GFAP antibody from other lab ( DAKO company product ) and followed the same procedures and make comparison with your company's GFAP. We obtained typical image of astrocyte by GFAP of borrowed antibody (DAKO product). However, for your company's GFAP, we obtained images similar to neurons just like before. Besides, for the MAP2 of your company, we cannot get any typical images of neurons although we followed the same procedures as doing GFAP. For every experiment, we made negative controls. Finally, we believe there must be somthing wrong with your two primary antibodies(GFAP and MAP2). It cost us more than two weeks to do such experiments which made us very frustrated. We just want to proceed our experiment quickly. so IF possible, someone can check it for us and send us the right antibody quickly so we can continue our experiments.

Read More

Abcam community

Verified customer

Asked on Nov 02 2011

Answer

We spoke just a short while ago regarding these issues you were having with ab90601 and ab11267. I have processed the replacement orders as we discussed and sent you an update via email just a few minutes ago. You should receive these 2 replacement antibodies for ab90601 and ab11267 tomorrow. I am sorry for the trouble these 2 antibodies have caused and I do hope the replacements work as expected. Please feel free to follow up with me if you need anything further.

Read More

Abcam Scientific Support

回复于 Nov 02 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2023 艾博抗(上海)贸易有限公司版权所有