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驴抗大鼠IgG H&L (Alexa Fluor® 647)预吸附二抗(ab150155)

Reviews (1) Submit a question References (51)
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150155)
  • Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150155)

Key features and details

  • Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, Flow Cyt, ELISA, IHC-P

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 680 Alexa Fluor® 750 Biotin DyLight® 488 DyLight® 550 DyLight® 650 FITC HRP PE Texas Red ® Unconjugated

概述

  • 产品名称

    驴抗大鼠IgG H&L (Alexa Fluor® 647)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Donkey

  • 靶标种属

    Rat

  • 特异性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

  • 经测试应用

    适用于: IHC-Fr, ICC/IF, Flow Cyt, ELISA, IHC-Pmore details

  • 最小交叉反应


    Human, Mouse more details

  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 偶联物

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 常规说明

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究领域

相关产品

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab150155于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr (1)
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
Flow Cyt
1/2000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
说明
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
Flow Cyt
1/2000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

图片

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150155)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150155)

    ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (red) was ab150155 Alexa Fluor® 647 donkey anti-rat IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150155)
    Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150155)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (51)

发表研究结果有使用 ab150155？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab150155 被引用在 51 文献中.

  • Amadei G  et al. Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro. Dev Cell 56:366-382.e9 (2021). PubMed: 33378662
  • Yang Y  et al. A modified tape transfer approach for rapidly preparing high-quality cryosections of undecalcified adult rodent bones. J Orthop Translat 26:92-100 (2021). PubMed: 33437628
  • Jin H  et al. Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury. Nat Commun 12:2863 (2021). PubMed: 34001904
  • Choudhury IN  et al. Antimicrobial responses of peripheral and central nervous system glia against Staphylococcus aureus. Sci Rep 11:10722 (2021). PubMed: 34021227
  • Pitsidianaki I  et al. Mesenchymal-to-epithelial transitions require tissue-specific interactions with distinct laminins. J Cell Biol 220:N/A (2021). PubMed: 34047771
View all Publications for this product

客户评价及客户问答

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Excellent results

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Adult mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS. Sections were incubated with either (A) no primary antibody or (B ) anti-TRA98 (Abcam ab82527) at 1:1000 dilution for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and Donkey-Anti Rat 647 (Abcam ab150155) applied at a 1:500 dilution. Sections were then mounted after washing 3X with 0.1% Triton X-100 in PBS.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Bryan Niedenberger

Verified customer

提交于 Jul 11 2016

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