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驴抗兔IgG H&L (HRP)预吸附二抗(ab7083)

Submit a review Q&A (3)References (19)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Fr

概述

  • 产品名称

    驴抗兔IgG H&L (HRP)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Donkey

  • 靶标种属

    Rabbit

  • 特异性

    No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

  • 经测试应用

    适用于: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details

  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • 免疫原

    Full length native Rabbit IgG (purified).

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 6.50
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Horseradish Peroxidase (HRP)

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7083于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/1000 - 1/3000.
IHC-P
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
ELISA
1/7500 - 1/30000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
说明
ICC/IF
1/1000 - 1/3000.
IHC-P
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
ELISA
1/7500 - 1/30000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (19)

发表研究结果有使用 ab7083？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7083 被引用在 19 文献中.

  • Alhawamdeh M  et al. Effect of IFN‑γ encapsulated liposomes on major signal transduction pathways in the lymphocytes of patients with lung cancer. Oncol Lett 27:8 (2024). PubMed: 38028180
  • Thongkorn S  et al. Investigation of autism-related transcription factors underlying sex differences in the effects of bisphenol A on transcriptome profiles and synaptogenesis in the offspring hippocampus. Biol Sex Differ 14:8 (2023). PubMed: 36803626
  • Lertpanprom M  et al. High expression of protein tyrosine phosphatase receptor S (PTPRS) is an independent prognostic marker for cholangiocarcinoma. Front Public Health 10:835914 (2022). PubMed: 35991009
  • Jiang S  et al. Hydrogen sulphide reduces hyperhomocysteinaemia-induced endothelial ER stress by sulfhydrating protein disulphide isomerase to attenuate atherosclerosis. J Cell Mol Med 25:3437-3448 (2021). PubMed: 33675119
  • Cui X  et al. MiR-302b-5p enhances the neuroprotective effect of IGF-1 in methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease by regulating inducible nitric-oxide synthase. Cell Biochem Funct N/A:N/A (2020). PubMed: 32474958
View all Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Again, thank you so much for the insight. I did the experiment using both adult rat hippocampus and 3-week-old pup liver against B-actin as control. Apparently, both proteins showed a weak bands, the former was expressed at around ˜35kDa and the latter at around ˜25kDa (which reflected the same results from the first experiment). Could it be that this is actually the folate binding protein (FR-alpha)? It would make sense that the adult rat had a higher kDa post glycosylation. Kindly see the attached file and give us your insight. Thank you so much! :)


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Answer

Thank you for contacting us again and sending this new information.

The WB image looks very convincing and proofs that the antibody is actually picking up its target as it should.

Regarding the double band in the pup sample it may be that the antibody is indeed picking up the non-glycosylated form. In addition, the double band may be due to a low glycosylation level or may be the protein chain plus the C-terminal pro-peptide (approx. 27 kDa).

I hope this information is again helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I truly appreciate your immediate reply especially at this time. From your suggestion, I will try to do a positive control run using a female adult hippocampus sample. Will that be okay? Regarding my inquiry, could you enlighten me on the expression at the lower band around ˜10kDa, whether it is a nonspecific band or is just part of my target protein that has undergone post translational modification? I appreciate if you clarify any of these.:)

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Answer

Thank you for your reply.

Unfortunately, it is quite hard to get some information if this protein expressed in the rat hippocampus. However, I checked the SwissProt site for more information regardingthis for the human protein, and it looks like in human it is only expressed in the cerebellum. I would therefore suggest rather to use a whole brain or cerebellum lysate in case the expression pattern is the same as in humans. Kidneys do also show a high expression level in rats according to UniGene.

To be honest, I cannot tell you if the signal at 10 kDa is specific or not. From checking the SwissProt side, I would say it is probably non-specific, but as I cannot find any information there for the rat protein, it is rather a guess than really knowing. In addition, UniGene reports anon-existing expression level in liver.

Sorry to disappoint here that I cannot help to solve the riddle at the moment.

Please let me know if the new samples provide the expected results.

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Question


I recently ordered Anti-Folate binding protein primary antibody (EPR4708) Cat # ab125030 and donkey anti-rabbit IgG H&L (HRP) secondary anitbody Cat # ab7083 to measure the expression of FBP1 (Folate receptor alpha) that has a predicted band size at 30kDa. The primary antibody I used is predicted to work with rat. I am using a 3-week-old Sprague Dawley rat pup liver sample and did the Western Blot experiment according to the Abcam protocols book (First edition). My B-actin turned out with a specific band at around 43kDa, however, for my target protein FR alpha, 2 nonspecific weak bands (4 columns respective for 4 sample groups) were seen at around 25kDa and 11kDa. Kindly checked the attached file and please let me know whether I can consider any of them as specific to my protein of interest. Thank you so much and hoping for your utmost response. Have a good day!



Sincerely,

Kay





Karen Kay Mejos
Dept. of Nutritional Science and Food Management
Ewha Womans University
11-1 Daehyun-dong, Seodaemun-ku
Seoul, 120-750 Korea
Tel: +82-2-3277-4426
Fax: +82-2-3277-2898
Mobile: 010-9073-9216
e-mail: karenmejos@ewhain.net










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Answer

Thank you for resending the image. Based on your description of the upper band as being ˜ 25kD it seems this could be the correct band, as the size can vary a few kD just based on the gel, buffer, etc. Adding a negative control could help ensure that this is not a non-specific band. Please let me know if you have any further questions.

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