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Immunology Immunoglobulins Heavy Chain IgG

驴抗兔IgG H&L (HRP) (ab6802)

  • Datasheet
  • SDS
Submit a review Q&A (4)References (103)

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Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

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概述

  • 产品名称

    驴抗兔IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 宿主

    Donkey
  • 靶标种属

    Rabbit
  • 经测试应用

    适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • 免疫原

    Rabbit IgG, whole molecule

  • 偶联物

    HRP

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Enzyme
    • HRP

相关产品

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6802于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/3000.
ELISA
1/160000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2000.
IHC-P
1/500 - 1/2000.
IHC-Fr
1/500 - 1/2000.
说明
WB
1/1000 - 1/3000.
ELISA
1/160000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2000.
IHC-P
1/500 - 1/2000.
IHC-Fr
1/500 - 1/2000.

图片

  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)Samson et al PLoS Genet. 2014 Oct 9;10(10):e1004588. doi: 10.1371/journal.pgen.1004588. eCollection 2014 Oct. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Canonical histones are retained in C. elegans sperm.

    A–E) Western blot analysis of sperm and embryo lysates loaded with equivalent amounts of DNA.
    For full details please see paper.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

    IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8227, 5 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6802, 1/2,000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)This image is courtesy of an anonymous Abreview.
  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)This image is courtesy of an Abreview submitted by Min young Kim

    See Abreview

  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)

    Lane M: 3 µl Molecular ladder.

    Lane 1: Rabbit IgG whole molecule.

    Lane 2: Rabbit IgG F(ab) fragment.

    Lane 3: Rabbit IgG F(c) fragment.

    Lane 4: Rabbit IgM whole molecule.

    Lane 5: Normal rabbit serum.

    All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.

    ab6802 used at 1:5,000 for 60 min at RT. Secondary antibody: None. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (103)

发表研究结果有使用 ab6802?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6802 被引用在 103 文献中.

  • Zhai Z  et al. MiR-181a-5p facilitates proliferation, invasion, and glycolysis of breast cancer through NDRG2-mediated activation of PTEN/AKT pathway. Bioengineered 13:83-95 (2022). PubMed: 34951340
  • Liu AL  et al. The role of ipRGCs in ocular growth and myopia development. Sci Adv 8:eabm9027 (2022). PubMed: 35675393
  • Son SH  et al. Structural and Functional Insights into CP2c Transcription Factor Complexes. Int J Mol Sci 23:N/A (2022). PubMed: 35742810
  • Liu ZM  et al. SP1 Promotes HDAC4 Expression and Inhibits HMGB1 Expression to Reduce Intestinal Barrier Dysfunction, Oxidative Stress, and Inflammatory Response after Sepsis. J Innate Immun 14:366-379 (2022). PubMed: 35780770
  • Skoug C  et al. Sphingosine 1-Phoshpate Receptors are Located in Synapses and Control Spontaneous Activity of Mouse Neurons in Culture. Neurochem Res 47:3114-3125 (2022). PubMed: 35781853
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-4 of 4 Abreviews or Q&A

Question

I recently bought this and found that the antibody solution is not clear, but is suspension. Is it normal and do I need to centrifuge it before use?

Read More

Abcam community

Verified customer

Asked on Jul 15 2015

Answer

It is normal that the solution is not completely clear. We recommend centrifuging the solution if it is still an unclear suspension after standing at room temperature (and gently mixed with some finger tapping).

Read More

Abcam Scientific Support

回复于 Jul 15 2015

Question

What is the recomended dilution factor for ab22696, ab54615, ab103724 and ab126709?

Read More

Abcam community

Verified customer

Asked on Sep 18 2012

Answer

Thank you for contacting us.

we tested catalog ab126709in IP, IHC, ICC, and Flow cytometry and it is tested negative. For this reason, we only recommending the product for western blot.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Sep 18 2012

Question

What is the recomended dilution factor for ab22696, ab54615, ab103724 and ab126709?

Read More

Abcam community

Verified customer

Asked on Sep 17 2012

Answer

Thank you for contacting us.

The ab22696 could be used at 1/10 - 1/100 dilution.
ab54615 at 5-10ug/ml
ab103724 at 5-10ug/ml
ab126709 at 1/500-1/1000 dilution.

ab6820 and ab6802 at 1/500-1/2000 dilution.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Sep 17 2012

Question

Hi Abcam,

I tried contacting the abcam distributer here in Australia (Sapphire bioscience), but the representative was unable to answer my questions.

I would like to purchase one secondary antibody against primary abcam antibodies I already have.

I would like to use the antibodies in a dot blot assay, using the ECL detection method.

There are three possible secondary antibodies that seem to me like they will work for two of my primaries:

Primary: ab8898 and ab9051
Secondary: ab97064, ab97095 and ab97200

Which secondary should I choose that will be best for both of my primaries, using ECL detection in a Dot Blot assay?

Thank you in advance for your assistance.

Read More

Abcam community

Verified customer

Asked on Mar 27 2012

Answer

Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not tested them specifically for this application and thus cannot guarantee that it would work.

Therefore, I would like to recommend one of the following secondaries which have been tested in dot blot:

ab6721: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html).

ab6802: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html).

ab6795: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html (or use the following: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html).

They are raised in goat, donkey and sheep and apart from this are equally well suited for your experimental setup. If you need a decision guiding you could for example choose the one with the most references: this would be ab6721 with 52 publications mentioning the use of this antibody.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (https://www.abcam.com/Abpromise).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

回复于 Mar 27 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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