驴抗兔IgG H&L (HRP) (ab6802)
Key features and details
- Donkey Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
概述
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产品名称
驴抗兔IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rabbit -
经测试应用
适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
免疫原
Rabbit IgG, whole molecule
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. -
克隆
多克隆 -
同种型
IgG -
研究领域
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Substrate reagent
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- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/3000.
|
|
| ELISA |
1/160000.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/500 - 1/2000.
|
|
| IHC-P |
1/500 - 1/2000.
|
|
| IHC-Fr |
1/500 - 1/2000.
|
| 说明 |
|---|
|
WB
1/1000 - 1/3000. |
|
ELISA
1/160000. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Dot blot
Use at an assay dependent concentration. |
|
ICC/IF
1/500 - 1/2000. |
|
IHC-P
1/500 - 1/2000. |
|
IHC-Fr
1/500 - 1/2000. |
图片
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Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)Samson et al PLoS Genet. 2014 Oct 9;10(10):e1004588. doi: 10.1371/journal.pgen.1004588. eCollection 2014 Oct. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Canonical histones are retained in C. elegans sperm.
A–E) Western blot analysis of sperm and embryo lysates loaded with equivalent amounts of DNA.
For full details please see paper. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8227, 5 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6802, 1/2,000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)This image is courtesy of an anonymous Abreview. -
Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)This image is courtesy of an Abreview submitted by Min young Kim -
Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab6802)
Lane M: 3 µl Molecular ladder.
Lane 1: Rabbit IgG whole molecule.
Lane 2: Rabbit IgG F(ab) fragment.
Lane 3: Rabbit IgG F(c) fragment.
Lane 4: Rabbit IgM whole molecule.
Lane 5: Normal rabbit serum.
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
ab6802 used at 1:5,000 for 60 min at RT. Secondary antibody: None. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (115)
ab6802 被引用在 115 文献中.
- Skoug C et al. Density of Sphingosine-1-Phosphate Receptors Is Altered in Cortical Nerve-Terminals of Insulin-Resistant Goto-Kakizaki Rats and Diet-Induced Obese Mice. Neurochem Res 49:338-347 (2024). PubMed: 37794263
- Al-Abedi R et al. Therapeutic Fractional Doses of Ionizing Radiation Promote Epithelial-Mesenchymal Transition, Enhanced Invasiveness, and Altered Glycosylation in MCF-7 Breast Cancer Cells. Genome Integr 14:2 (2023). PubMed: 38025522
- Zhao YM et al. Intravitreal slow-release dexamethasone alleviates traumatic proliferative vitreoretinopathy by inhibiting persistent inflammation and Müller cell gliosis in rabbits. Int J Ophthalmol 16:22-32 (2023). PubMed: 36659954
- Wang R et al. ACTN1 interacts with ITGA5 to promote cell proliferation, invasion and epithelial-mesenchymal transformation in head and neck squamous cell carcinoma. Iran J Basic Med Sci 26:200-207 (2023). PubMed: 36742137
- Yinhua Zhao MM et al. Extracellular vesicles from hypoxia-pretreated adipose-derived stem cells regulate hypoxia/reoxygenation-induced human dermal microvascular endothelial apoptosis and autophagy in vitro. Heliyon 9:e13315 (2023). PubMed: 36747525