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驴抗兔IgG H&L (FITC)预吸附二抗(ab7079)

Submit a review Q&A (2)References (3)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Donkey
  • Suitable for: WB, ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA

概述

  • 产品名称

    驴抗兔IgG H&L (FITC)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Donkey

  • 靶标种属

    Rabbit

  • 特异性

    No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

  • 经测试应用

    适用于: WB, ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details

  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • 免疫原

    Full length native Rabbit IgG (purified).

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7079于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
ICC/IF
1/1000 - 1/5000.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
ICC/IF
1/1000 - 1/5000.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (3)

发表研究结果有使用 ab7079？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7079 被引用在 3 文献中.

  • Griner SL  et al. Structure-based inhibitors of amyloid beta core suggest a common interface with tau. Elife 8:N/A (2019). PubMed: 31612856
  • Singh SJ  et al. Metabolic shift in density-dependent stem cell differentiation. Cell Commun Signal 15:44 (2017). PubMed: 29052507
  • Guo Z  et al. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells. J Immunol 190:4337-48 (2013). PubMed: 23514739

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Dear xxxxxx,

Any chemical (for eg: glycerol) should be added to it when we aliquot it for freezing?

Thank you for your prompt reply.

Best regards,

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Answer

Thank you for your reply.

Addition of glycerol to the antibody should not be required to maintain the activity of the antibody, however, if the customer wants to add glycerol please make them aware that glycerol can be contaminated with bacteria. If adding glycerol or any cryoprotectant, care should be taken to obtain a sterile preparation. Storing solutions containing glycerol at -80oC is not advised since this is below the freezing point of glycerol.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

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Question

Dear Technical support,

Can I get your advice on the storage method of this Donkey anti-Rabbit IgG H&L (FITC) secondary antibody" with cat number: ab7079?

It is a FITC conjugated secondary antibody. The datasheet of the product recommend it to be store at -20°C or -80°C.

Conjugated antibody stable to be store at -20C or -80C ? Freezing and thawing will not affect the conjugation?

Thank you. We look forward to hearing from you soon.

Best regards,

Read More
Answer

Thank you for contacting us and your interest in our products.

We would advise storing the Donkey anti-Rabbit IgG H&L (FITC) secondary antibody (ab7079) at +4°C short term (1-2 weeks). For longer term storage we would advise storing the antibody at -20°C or -80°C.We would advise avoid repeated freeze / thaw cycles as far as possible as this can affect the activity of antibodies. We would therefore recommend aliguoting the antibody before freezing.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

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