驴抗兔IgG H&L (DyLight® 650)预吸附二抗(ab96922)
Key features and details
- Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Flow Cyt
Related conjugates and formulations
概述
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产品名称
驴抗兔IgG H&L (DyLight® 650)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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经测试应用
适用于: ICC/IF, IHC-P, Flow Cytmore details -
最小交叉反应
Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details -
偶联物
DyLight® 650. Ex: 654nm, Em: 673nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
克隆
多克隆 -
同种型
IgG -
常规说明
DyLight® 650 replaces DyLight® 649. -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96922于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/500.
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| IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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| Flow Cyt |
1/50 - 1/200.
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| 说明 |
|---|
|
ICC/IF
1/50 - 1/500. |
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IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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Flow Cyt
1/50 - 1/200. |
图片
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Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96922)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (12)
ab96922 被引用在 12 文献中.
- Hamade H et al. BATF3 Protects Against Metabolic Syndrome and Maintains Intestinal Epithelial Homeostasis. Front Immunol 13:841065 (2022). PubMed: 35812447
- Moser B et al. The inflammatory kinase IKKa phosphorylates and stabilizes c-Myc and enhances its activity. Mol Cancer 20:16 (2021). PubMed: 33461590
- Neddens J et al. Correlation of pyroglutamate amyloid ß and ptau Ser202/Thr205 levels in Alzheimer's disease and related murine models. PLoS One 15:e0235543 (2020). PubMed: 32645028
- Tan EE et al. Dominant-negative NFKBIA mutation promotes IL-1ß production causing hepatic disease with severe immunodeficiency. J Clin Invest 130:5817-5832 (2020). PubMed: 32750042
- Neddens J et al. Constant Levels of Tau Phosphorylation in the Brain of htau Mice. Front Mol Neurosci 13:136 (2020). PubMed: 32982685
