驴抗兔IgG H&L (DyLight® 594)预吸附二抗(ab96921)
Key features and details
- Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed
- Conjugation: DyLight® 594. Ex: 593nm, Em: 618nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P
Related conjugates and formulations
概述
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产品名称
驴抗兔IgG H&L (DyLight® 594)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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经测试应用
适用于: Flow Cyt, ICC/IF, IHC-Pmore details -
最小交叉反应
Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details -
偶联物
DyLight® 594. Ex: 593nm, Em: 618nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96921于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
1/50 - 1/200.
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ICC/IF |
1/50 - 1/500.
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IHC-P |
1/50 - 1/500.
|
说明 |
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Flow Cyt
1/50 - 1/200. |
ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
图片
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Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed (ab96921)
E14 mouse brain sample was stained with primary antibody Rabbit anti-Clim1. The secondary antibody used was ab96921 (1:200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.
This image is courtesy of an anonymous Abreview
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab96921 被引用在 18 文献中.
- Liu J et al. Increased alveolar epithelial TRAF6 via autophagy-dependent TRIM37 degradation mediates particulate matter-induced lung metastasis. Autophagy 18:971-989 (2022). PubMed: 34524943
- Xiu H et al. Neddylation Alleviates Methicillin-Resistant Staphylococcus aureus Infection by Inducing Macrophage Reactive Oxygen Species Production. J Immunol 207:296-307 (2021). PubMed: 34183370
- Rajendran A et al. Tissue-specific deletion of mouse basolateral uniporter LAT4 (Slc43a2) reveals its crucial role in small intestine and kidney amino acid transport. J Physiol 598:5109-5132 (2020). PubMed: 32841365
- Dolgodilina E et al. Choroid plexus LAT2 and SNAT3 as partners in CSF amino acid homeostasis maintenance. Fluids Barriers CNS 17:17 (2020). PubMed: 32046769
- Oparija-Rogenmozere L et al. Phosphorylation of mouse intestinal basolateral amino acid uniporter LAT4 is controlled by food-entrained diurnal rhythm and dietary proteins. PLoS One 15:e0233863 (2020). PubMed: 32470053