驴抗兔IgG H&L (Biotin) (ab6801)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
概述
-
产品名称
驴抗兔IgG H&L (Biotin)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rabbit -
经测试应用
适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
免疫原
Rabbit IgG whole molecule
-
偶联物
Biotin
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule. -
共轭说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/3000 - 1/15000.
|
|
| ELISA |
1/150000.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/500 - 1/3000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/3000 - 1/15000. |
|
ELISA
1/150000. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Dot blot
Use at an assay dependent concentration. |
|
ICC/IF
1/500 - 1/3000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
图片
-
Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (Biotin) (ab6801)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (Biotin) (ab6801)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (12)
ab6801 被引用在 12 文献中.
- Russell JP et al. Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells. Elife 10:N/A (2021). PubMed: 33399538
- Ding Q et al. miR-126 promotes the growth and proliferation of leukemia stem cells by targeting DNA methyltransferase 1. Int J Clin Exp Pathol 11:3454-3462 (2018). PubMed: 31949723
- Johnson MB et al. Topical Fibronectin Improves Wound Healing of Irradiated Skin. Sci Rep 7:3876 (2017). IHC-P ; Mouse . PubMed: 28634413
- Chaurasiya S et al. Breast cancer gene therapy using an adenovirus encoding human IL-2 under control of mammaglobin promoter/enhancer sequences. Cancer Gene Ther 23:178-87 (2016). PubMed: 27151235
- Latorre R et al. Expression of the Bitter Taste Receptor, T2R38, in Enteroendocrine Cells of the Colonic Mucosa of Overweight/Obese vs. Lean Subjects. PLoS One 11:e0147468 (2016). PubMed: 26866366