驴抗兔IgG H&L (Alkaline Phosphatase)预吸附二抗(ab97082)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, WB, IHC-P, ELISA
概述
-
产品名称
驴抗兔IgG H&L (Alkaline Phosphatase)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
-
经测试应用
适用于: ICC, WB, IHC-P, ELISAmore details -
最小交叉反应
Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details -
免疫原
Other Immunogen Type corresponding to Rabbit IgG.
-
偶联物
Alkaline Phosphatase
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97082于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
|
|
| WB |
1/2500 - 1/25000. (Chemiluminescent)
|
|
| IHC-P |
1/100 - 1/1000.
|
|
| ELISA |
1/2500 - 1/25000. (Primary ELISA)
|
| 说明 |
|---|
|
ICC
Use at an assay dependent dilution. |
|
WB
1/2500 - 1/25000. (Chemiluminescent) |
|
IHC-P
1/100 - 1/1000. |
|
ELISA
1/2500 - 1/25000. (Primary ELISA) |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (3)
ab97082 被引用在 3 文献中.
- Matsuoka T et al. Inhibition of NLRP3 inflammasome by MCC950 improves the metabolic outcome of islet transplantation by suppressing IL-1ß and islet cellular death. Sci Rep 10:17920 (2020). PubMed: 33087823
- Rigon L et al. Targeting Brain Disease in MPSII: Preclinical Evaluation of IDS-Loaded PLGA Nanoparticles. Int J Mol Sci 20:N/A (2019). PubMed: 31022913
- Ligaba-Osena A et al. Reducing biomass recalcitrance by heterologous expression of a bacterial peroxidase in tobacco (Nicotiana benthamiana). Sci Rep 7:17104 (2017). WB . PubMed: 29213132
