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驴抗小鼠IgG H&L (HRP)预吸附二抗(ab7061)

Submit a review Q&A (2)References (8)

Key features and details

  • Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Donkey
  • Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm PE Texas Red ® TRITC Unconjugated Unconjugated

概述

  • 产品名称

    驴抗小鼠IgG H&L (HRP)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Donkey

  • 靶标种属

    Mouse

  • 特异性

    No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

  • 经测试应用

    适用于: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details

  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native mouse IgG (purified).

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    Mouse IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Horseradish Peroxidase (HRP)

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7061于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/2000 - 1/20000.
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent concentration.
ELISA
1/20000 - 1/80000.
IHC-P
1/1000 - 1/5000.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
说明
WB
1/2000 - 1/20000.
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent concentration.
ELISA
1/20000 - 1/80000.
IHC-P
1/1000 - 1/5000.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (8)

发表研究结果有使用 ab7061？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7061 被引用在 8 文献中.

  • Nakai A  et al. Crucial role of TFAP2B in the nervous system for regulating NREM sleep. Mol Brain 17:13 (2024). PubMed: 38413970
  • Oh S  et al. Precision targeting of autoantigen-specific B cells in muscle-specific tyrosine kinase myasthenia gravis with chimeric autoantibody receptor T cells. Nat Biotechnol 41:1229-1238 (2023). PubMed: 36658341
  • Jiang S  et al. Hydrogen sulphide reduces hyperhomocysteinaemia-induced endothelial ER stress by sulfhydrating protein disulphide isomerase to attenuate atherosclerosis. J Cell Mol Med 25:3437-3448 (2021). PubMed: 33675119
  • Jermusyk A  et al. A 584 bp deletion in CTRB2 inhibits chymotrypsin B2 activity and secretion and confers risk of pancreatic cancer. Am J Hum Genet 108:1852-1865 (2021). PubMed: 34559995
  • Fan J  et al. Hydrogen sulfide lowers hyperhomocysteinemia dependent on cystathionine ? lyase S-sulfhydration in ApoE-knockout atherosclerotic mice. Br J Pharmacol 176:3180-3192 (2019). PubMed: 31140595
  • Li L  et al. Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy. Exp Ther Med 18:4313-4321 (2019). PubMed: 31777538
  • Kolyada A  et al. Soluble analog of ApoER2 targeting beta2-glycoprotein I in immune complexes counteracts hypertension in lupus-prone mice with spontaneous antiphospholipid syndrome. J Thromb Haemost 14:1298-307 (2016). PubMed: 26990752
  • Kolyada A  et al. Inhibition of thrombotic properties of persistent autoimmune anti-ß2GPI antibodies in the mouse model of antiphospholipid syndrome. Blood 123:1090-7 (2014). PubMed: 24277078

客户评价及客户问答

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Question

I am looking for a HRP conjugated anti-mouse antibody to be used in ELISA. The most important thing is that it will have the least cross reactivity to rat immunoglobulins. I see that you have many such antibodies. Which one would you think is the most suitable?


Thanks,

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Answer

Thank you for contacting us.

The following products would be suitable as per your requirements. The catalogue numbers are

ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

1. Details of preabsorbtion information of secondary ab7061. 2. Details of dilution range. Persoxidase activity block

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Answer

Thank you for your enquiry. I am sorry to hear that you have been obtaining high background with this antibody. Following contact with the source of this antibody I have determined that the antibody is affinity purified, then cross adsorbed using solid phase adsorption. This means the affinity purified antibody is passed through column(s) bound with listed species of IgGs. The antibodies which cross react with the listed IgGs will bind to the column(s), thus being removed from the final product. The major background in IHC and FACS is from Fc receptors on the cell surface. I think the best way around this problem is to use antibodies specific to the F(ab')2 fragment instead of whole IgG. e.g. ab5766 Mouse IgG antibody H&L - F(ab)2 Fragment Texas Red conjugated, Goat, FACS, IM. I do also think that it is worth inhibiting the peroxidase activity of this enzyme to ensure that the signal that you are observing is not due to the HRP substrate reacting with the endogenous peroxidase activity. I have determined from the source of this antibody that a good starting dilution is 1:1000. I hope this information helps, please do not hesitate to contact me should you require further assistance.

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