驴抗小鼠IgG H&L (HRP) (ab6820)
Key features and details
- Donkey Anti-Mouse IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WB, ICC/IF
概述
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产品名称
驴抗小鼠IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Mouse -
经测试应用
适用于: Dot blot, ELISA, IHC-P, IHC-Fr, WB, ICC/IFmore details -
免疫原
mouse IgG whole molecule
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. -
共轭说明
Horseradish Peroxidase (HRP) -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
应用 | Ab评论 | 说明 |
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Dot blot |
Use at an assay dependent concentration.
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ELISA |
1/70000.
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IHC-P |
1/500 - 1/2000.
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IHC-Fr |
1/500 - 1/2000.
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|
WB |
1/2000 - 1/10000.
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|
ICC/IF |
1/500 - 1/2500.
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说明 |
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Dot blot
Use at an assay dependent concentration. |
ELISA
1/70000. |
IHC-P
1/500 - 1/2000. |
IHC-Fr
1/500 - 1/2000. |
WB
1/2000 - 1/10000. |
ICC/IF
1/500 - 1/2500. |
图片
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Western blot - Donkey Anti-Mouse IgG H&L (HRP) (ab6820)Portegijs et al PLoS Genet. 2016 Oct 6;12(10):e1006291. doi: 10.1371/journal.pgen.1006291. eCollection 2016 Oct. Fig S5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Detection of protein levels in LIN-5 mutants.
(Panel A) Western blots of lysates of yeast clones containing the indicated LIN-5 expression constructs, probed for LIN-5 and tubulin (loading control) levels.
Protein samples were separated on gradient acrylamide gels and subjected to western blotting on polyvinylidene difluoride membrane. Membranes were blocked with 5% skim milk in PBST for 1 hour at room temperature, or overnight at 4°C for stripped blots. For protein detection, primary antibodies used in this study were: mouse anti–LIN-5 (1:1000) and rabbit anti-Tubulin (1:1000, Abcam) for stripped blots. Secondary antibodies used were: donkey anti-mouse HRP (1:5000, Abcam, ab6820) and goat anti-rabbit HRP (1:5000).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (HRP) (ab6820)
IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6820, 1/2000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (41)
ab6820 被引用在 41 文献中.
- Klarić ML et al. FANCM Gene Variants in a Male Diagnosed with Sertoli Cell-Only Syndrome and Diffuse Astrocytoma. Genes (Basel) 15:N/A (2024). PubMed: 38927643
- Horton JR et al. A complete methyl-lysine binding aromatic cage constructed by two domains of PHF2. J Biol Chem 299:102862 (2023). PubMed: 36596360
- Vo T et al. Spatial transcriptomic analysis of Sonic hedgehog medulloblastoma identifies that the loss of heterogeneity and promotion of differentiation underlies the response to CDK4/6 inhibition. Genome Med 15:29 (2023). PubMed: 37127652
- Chen Q et al. GSK-3484862 targets DNMT1 for degradation in cells. NAR Cancer 5:zcad022 (2023). PubMed: 37206360
- Li Y et al. Single-cell m6A mapping in vivo using picoMeRIP-seq. Nat Biotechnol N/A:N/A (2023). PubMed: 37349523