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驴抗小鼠IgG H&L (DyLight® 650) (ab96878)

Submit a review Q&A (1)References (3)
Donkey Anti-Mouse IgG H&L (DyLight® 650) (ab96878)

    Key features and details

    • Donkey Anti-Mouse IgG H&L (DyLight® 650)
    • Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: IHC-P, ICC/IF, Flow Cyt

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 FITC Gold 10nm Gold 12nm Gold 18nm Gold 6nm HRP PE Texas Red ® TRITC Unconjugated Unconjugated

    概述

    • 产品名称

      驴抗小鼠IgG H&L (DyLight® 650)
      参阅全部 IgG 二抗

    • 宿主

      Donkey

    • 靶标种属

      Mouse

    • 特异性

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

    • 经测试应用

      适用于: IHC-P, ICC/IF, Flow Cytmore details

    • 偶联物

      DyLight® 650. Ex: 654nm, Em: 673nm

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 纯化说明

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 常规说明

      DyLight® 650 replaces DyLight® 649.

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab96878于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/50 - 1/500.

    DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.
    说明
    IHC-P
    1/50 - 1/500.

    DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.

    图片

    • Donkey Anti-Mouse IgG H&L (DyLight® 650) (ab96878)
      Donkey Anti-Mouse IgG H&L (DyLight® 650) (ab96878)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (3)

    发表研究结果有使用 ab96878？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab96878 被引用在 3 文献中.

    • Manirujjaman M  et al. Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy. Cells 9:N/A (2020). PubMed: 31952347
    • Tanaka K  et al. Combined effect of canagliflozin and exercise training on high-fat diet-fed mice. Am J Physiol Endocrinol Metab 318:E492-E503 (2020). PubMed: 32017594
    • Koch JC  et al. Alpha-Synuclein affects neurite morphology, autophagy, vesicle transport and axonal degeneration in CNS neurons. Cell Death Dis 6:e1811 (2015). PubMed: 26158517

    客户评价及客户问答

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    Question

    Hi,

    I have reviewed this profile. I might not clarify clearly what I need. I need to have the spectrum of the dye as you may know the excitation spectrum may cover a certain range not only at a certain point. I am doing multiple staining of different proteins so the overlap of either excitation or emission affect the result a lot. Can you please give me more details of your dye? (such as dye light). Thanks a lot!

    all the best

    Read More
    Answer

    Thank you for your reply. My colleague is not in the office today and that is the reason I am writing to you.

    I ma sorry there was a misunderstanding.

    We do have information on the spectra of DyLight® fluorochromes on our homepage:

    https://www.abcam.com/index.html?pageconfig=resource&rid=12934

    The spectra can be reviewed on the bottom of the page. Unfortunately, we do not have a spectra viewer where multiple dyes can be combined and tested for overlapping. I am sorry if this causes any inconvenience.

    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Maybe it is worth checking their website for a spectra viewer also.

    I hope this information is helpful and wish you good luck with your experiments.

    Read More

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