驴抗小鼠IgG H&L (DyLight® 488) (ab96875)
Key features and details
- Donkey Anti-Mouse IgG H&L (DyLight® 488)
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Flow Cyt
Related conjugates and formulations
概述
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产品名称
驴抗小鼠IgG H&L (DyLight® 488)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
经测试应用
适用于: ICC/IF, IHC-P, Flow Cytmore details -
免疫原
Other Immunogen Type corresponding to Mouse IgG.
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偶联物
DyLight® 488. Ex: 493nm, Em: 518nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96875于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/50 - 1/500.
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IHC-P |
1/50 - 1/500.
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|
Flow Cyt |
1/50 - 1/200.
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说明 |
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ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
图片
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)This image is courtesy of an Abreview submitted by Charles Pallangyo.
ab7817 staining alpha smooth muscle actin in mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hour at 25°C. A DyLight® 488-conjugated donkey anti-mouse IgG H&L (ab96875) (1/1000) was used as the secondary antibody. Vimentin appears in red.
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab96875 被引用在 17 文献中.
- Jamalpoor A et al. A novel human stem cell-based biomarker assay for in vitro assessment of developmental toxicity. Birth Defects Res 114:1210-1228 (2022). PubMed: 35289129
- Chen CF et al. Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton's Jelly. Int J Mol Sci 22:N/A (2021). PubMed: 34445692
- Dolgodilina E et al. Choroid plexus LAT2 and SNAT3 as partners in CSF amino acid homeostasis maintenance. Fluids Barriers CNS 17:17 (2020). PubMed: 32046769
- Jara C et al. Microencapsulation of cellular aggregates composed of differentiated insulin and glucagon-producing cells from human mesenchymal stem cells derived from adipose tissue. Diabetol Metab Syndr 12:66 (2020). PubMed: 32774470
- Sahasrabudhe A et al. Cyfip1 Regulates SynGAP1 at Hippocampal Synapses. Front Synaptic Neurosci 12:581714 (2020). PubMed: 33613257