驴抗小鼠IgG H&L (Alexa Fluor® 790)预吸附二抗(ab186699)
Key features and details
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) preadsorbed
- Conjugation: Alexa Fluor® 790. Ex: 782nm, Em: 805nm
- Host species: Donkey
- Suitable for: WB
Related conjugates and formulations
概述
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产品名称
驴抗小鼠IgG H&L (Alexa Fluor® 790)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species. -
经测试应用
适用于: WBmore details -
最小交叉反应
Chicken, Cow, Goat, Human, Rabbit, Rat, Sheep more details -
免疫原
The details of the immunogen for this antibody are not available.
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偶联物
Alexa Fluor® 790. Ex: 782nm, Em: 805nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using bovine, chicken, goat, human, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
克隆
多克隆 -
常规说明
We batch test Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) preadsorbed, ab186699 in fluorescent WB. Although we don’t batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success usingDonkey Anti-Mouse IgG H&L (Alexa Fluor® 790) preadsorbed, ab186699 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab186699于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/10000.
|
| 说明 |
|---|
|
WB
1/10000. |
图片
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Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) preadsorbed (ab186699)Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) preadsorbed (ab186699) at 1/10000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab186699 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) preadsorbed (ab186699)This image is courtesy of an anonymous AbreviewAll lanes : Mouse anti-beta actin
All lanes : 786-O whole cell lysate
Secondary
All lanes : Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) preadsorbed (ab186699)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab186699 被引用在 3 文献中.
- Wu Y et al. The Role of Sorting Nexin 17 in Cardiac Development. Front Cardiovasc Med 8:748891 (2021). PubMed: 34988124
- Childress ES et al. [1,2,5]Oxadiazolo[3,4-b]pyrazine-5,6-diamine Derivatives as Mitochondrial Uncouplers for the Potential Treatment of Nonalcoholic Steatohepatitis. J Med Chem 63:2511-2526 (2020). PubMed: 32017849
- Taylor BK et al. Opioid receptors inhibit the spinal AMPA receptor Ca2+ permeability that mediates latent pain sensitization. Exp Neurol 314:58-66 (2019). PubMed: 30660616