驴抗小鼠IgG H&L (Alexa Fluor® 488) (ab150105)
Key features and details
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488)
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, IHC-P, ELISA, Flow Cyt
Related conjugates and formulations
概述
-
产品名称
驴抗小鼠IgG H&L (Alexa Fluor® 488)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Mouse -
经测试应用
适用于: IHC-Fr, ICC/IF, IHC-P, ELISA, Flow Cytmore details -
免疫原
The details of the immunogen for this antibody are not available.
-
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
克隆
多克隆 -
同种型
IgG -
常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
-
研究领域
相关产品
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150105于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ICC/IF | (2) |
1/200 - 1/1000.
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| ELISA |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
1/2000.
ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody. |
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
1/200 - 1/1000. |
|
IHC-P
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
Flow Cyt
1/2000. ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody. |
图片
-
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150105 Alexa Fluor® 488 donkey anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
-
Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (187)
ab150105 被引用在 187 文献中.
- Kim J & Cooper JA Junctional Localization of Septin 2 Is Required for Organization of Junctional Proteins in Static Endothelial Monolayers. Arterioscler Thromb Vasc Biol 41:346-359 (2021). PubMed: 33147991
- Bojanowski K et al. Isosorbide Di-(Linoleate/Oleate) Stimulates Prodifferentiation Gene Expression to Restore the Epidermal Barrier and Improve Skin Hydration. J Invest Dermatol 141:1416-1427.e12 (2021). PubMed: 33181142
- Gunjigake K et al. Interleukin-17A derived from mast cells contributes to fibrosis in gastric cancer with peritoneal dissemination. Gastric Cancer 24:31-44 (2021). PubMed: 32488650
- Hosoya M et al. The common marmoset as suitable nonhuman alternative for the analysis of primate cochlear development. FEBS J 288:325-353 (2021). PubMed: 32323465
- Vitiello PP et al. Vulnerability to low-dose combination of irinotecan and niraparib in ATM-mutated colorectal cancer. J Exp Clin Cancer Res 40:15 (2021). PubMed: 33407715