驴抗小鼠IgG H&L (Alexa Fluor® 405)预吸附二抗(ab175659)
Key features and details
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ELISA, IHC-P, Flow Cyt, ICC/IF, IHC-Fr
概述
-
产品名称
驴抗小鼠IgG H&L (Alexa Fluor® 405)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species. -
经测试应用
适用于: ELISA, IHC-P, Flow Cyt, ICC/IF, IHC-Frmore details -
最小交叉反应
Chicken, Cow, Goat, Human, Rabbit, Rat, Sheep more details -
免疫原
The details of the immunogen for this antibody are not available.
-
偶联物
Alexa Fluor® 405. Ex: 402nm, Em: 421nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using bovine, chicken, goat, human, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
克隆
多克隆 -
同种型
IgG -
常规说明
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
-
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175659于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
Flow Cyt |
1/2000.
|
|
ICC/IF |
1/200 - 1/1000.
We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
|
IHC-Fr |
Use at an assay dependent concentration.
|
说明 |
---|
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
1/2000. |
ICC/IF
1/200 - 1/1000. We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
IHC-Fr
Use at an assay dependent concentration. |
图片
-
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175659, Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed, used at 1µg/ml for 1h at room temperature. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a dilution of 1/4000.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (8)
ab175659 被引用在 8 文献中.
- Yuan PQ et al. New insight on the enteric cholinergic innervation of the pig colon by central and peripheral nervous systems: reduction by repeated loperamide administration. Front Neurosci 17:1204233 (2023). PubMed: 37650102
- Wilson SB et al. DevKidCC allows for robust classification and direct comparisons of kidney organoid datasets. Genome Med 14:19 (2022). PubMed: 35189942
- Djenoune L et al. Autonomous Calcium Signaling in Human and Zebrafish Podocytes Controls Kidney Filtration Barrier Morphogenesis. J Am Soc Nephrol 32:1697-1712 (2021). PubMed: 33911000
- Kitanishi T et al. Robust information routing by dorsal subiculum neurons. Sci Adv 7:N/A (2021). PubMed: 33692111
- Han S et al. Proneural genes define ground-state rules to regulate neurogenic patterning and cortical folding. Neuron 109:2847-2863.e11 (2021). PubMed: 34407390
- Mulholland HN et al. Tightly coupled inhibitory and excitatory functional networks in the developing primary visual cortex. Elife 10:N/A (2021). PubMed: 34878404
- Nomura K et al. All-Electrical Ca2+-Independent Signal Transduction Mediates Attractive Sodium Taste in Taste Buds. Neuron 106:816-829.e6 (2020). PubMed: 32229307
- Heusinger J et al. Sensory deafferentation modulates and redistributes neurocan in the rat auditory brainstem. Brain Behav 9:e01353 (2019). PubMed: 31271523