驴抗小鼠IgG H&L (ab182022)
Key features and details
- Donkey Anti-Mouse IgG H&L
- Host species: Donkey
- Isotype: IgG
- Suitable for: ELISA, IHC-P, WB, IP
Related conjugates and formulations
概述
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产品名称
驴抗小鼠IgG H&L
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Mouse -
特异性
The antibody reacts with mouse immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Human IgG, rabbit IgG, goat IgG and Chicken IgY, less than 2%. Rat IgG, less than 33%. -
经测试应用
适用于: ELISA, IHC-P, WB, IPmore details -
免疫原
The details of the immunogen for this antibody are not available.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182022于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
1/20000 - 1/200000.
|
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| IHC-P |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
1/20000 - 1/200000. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
图片
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ELISA - Donkey Anti-Mouse IgG H&L (ab182022)
Cross-reactivity of the polyclonal secondary antibody ab182022 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182022 was then added starting at 1 µg/ml and gradually diluted 1:4 (50µl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1:20,000 dilution (50µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182022 showed a cross-reactivity below 2% towards all rabbit IgG, human IgG, goat IgG and chicken IgY and 33% towards rat IgG.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab182022 被引用在 1 文献中.
- Hauptman D et al. Leydig Cells in Patients with Non-Obstructive Azoospermia: Do They Really Proliferate? Life (Basel) 11:N/A (2021). PubMed: 34833142