驴抗山羊IgG H&L (PE)预吸附二抗(ab7004)
Key features and details
- Donkey Anti-Goat IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Dot blot, ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt
Related conjugates and formulations
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FITC
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HRP
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Unconjugated
Unconjugated
概述
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产品名称
驴抗山羊IgG H&L (PE)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Goat -
特异性
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, or Rat Serum Proteins.
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经测试应用
适用于: Dot blot, ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details -
最小交叉反应
Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat more details -
免疫原
Goat IgG whole molecule
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偶联物
PE. Ex: 488nm, Em: 575nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 -
克隆
多克隆 -
同种型
IgG -
常规说明
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
Use at an assay dependent concentration.
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| ICC/IF |
1/100 - 1/250.
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| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| Immunomicroscopy |
Use at an assay dependent concentration.
As a general guideline, dilutions of 1/50 to 1/200 should be suitable for most applications. |
|
| Flow Cyt | (1) |
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.
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| 说明 |
|---|
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Dot blot
Use at an assay dependent concentration. |
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ICC/IF
1/100 - 1/250. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
|
Immunomicroscopy
Use at an assay dependent concentration. As a general guideline, dilutions of 1/50 to 1/200 should be suitable for most applications. |
|
Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
图片
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Dot Blot - Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)
Dot Blot of ab7004.
Antigen: Goat IgG.
Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng, or 1.23 ng as indicated.
Secondary antibody: ab7004 at 1:1,000 for 60 min at RT.
Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT.
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Flow Cytometry - Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)Verified CustomerVery bright secondary antibody and PE is one of the best if you have a weak antigen. I used this in a perfect pairing with FITC in a FACS experiment to check pluripotency markers in human embryonic stem cells.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab7004 被引用在 9 文献中.
- Zumbrun EE et al. SARS-CoV-2 Aerosol and Intranasal Exposure Models in Ferrets. Viruses 15:N/A (2023). PubMed: 38140582
- Kim M et al. A Recombinant Ig Fragment (IgCw-?e?) Comprising the C?1-Ce2-4 and C? Domains Is an Alternative Reagent to Human IgE. J Immunol 208:772-779 (2022). PubMed: 35022271
- Arbore G et al. Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism. Nat Commun 9:4186 (2018). PubMed: 30305631
- Lan P et al. TNF superfamily receptor OX40 triggers invariant NKT cell pyroptosis and liver injury. J Clin Invest 127:2222-2234 (2017). PubMed: 28436935
- Mishra A et al. Mechanism, Consequences, and Therapeutic Targeting of Abnormal IL15 Signaling in Cutaneous T-cell Lymphoma. Cancer Discov 6:986-1005 (2016). PubMed: 27422033
- Rué L et al. Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels. J Clin Invest 126:4319-4330 (2016). PubMed: 27721240
- Chen SH et al. Umbilical cord blood-derived CD34? cells improve outcomes of traumatic brain injury in rats by stimulating angiogenesis and neurogenesis. Cell Transplant 23:959-79 (2014). PubMed: 23582375
- Williams JW et al. Transcription factor IRF4 drives dendritic cells to promote Th2 differentiation. Nat Commun 4:2990 (2013). PubMed: 24356538
- Miles A et al. CCL25 and CCL28 promote alpha4 beta7-integrin-dependent adhesion of lymphocytes to MAdCAM-1 under shear flow. Am J Physiol Gastrointest Liver Physiol 294:G1257-67 (2008). PubMed: 18308860