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Immunology Immunoglobulins Heavy Chain IgG

驴抗山羊IgG H&L (HRP) (ab97110)

  • Datasheet
  • SDS
Reviews (4)Q&A (2)References (92)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)

Key features and details

  • Donkey Anti-Goat IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-P, ELISA, WB, ICC

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概述

  • 产品名称

    驴抗山羊IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 宿主

    Donkey
  • 靶标种属

    Goat
  • 特异性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • 经测试应用

    适用于: IHC-P, ELISA, WB, ICCmore details
  • 免疫原

    Other Immunogen Type corresponding to Goat IgG. Contains both the heavy and light chain

  • 偶联物

    HRP

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 6.8
    Constituent: 0.05% CMIT/MIT based preservative
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 共轭说明

    Molar enzyme/ antibody protein ratio is 4:1
  • 克隆

    多克隆
  • 同种型

    IgG
  • 常规说明

    Part of the AbExcel range.
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Enzyme
    • HRP

相关产品

  • Related Products

    • Normal Donkey Serum (ab7475)
  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab97110于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/200 - 1/500.
ELISA (1)
1/10000 - 1/100000. (Primary).
WB (3)
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
ICC
1/200 - 1/500.
说明
IHC-P
1/200 - 1/500.
ELISA
1/10000 - 1/100000. (Primary).
WB
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
ICC
1/200 - 1/500.

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)

    IHC image of beta actin staining in human normal colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8229, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97110, 1/100 dilution) was used for 1 hour at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/200 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
    Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
    All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml

    All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 & 5-6 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/20000 dilution
    Lanes 3-4 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 20 minutes

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (92)

发表研究结果有使用 ab97110?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab97110 被引用在 92 文献中.

  • Weyant KB  et al. A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens. Nat Commun 14:464 (2023). PubMed: 36709333
  • Xiao Y  et al. CDK4/6 inhibitor palbociclib promotes SARS-CoV-2 cell entry by down-regulating SKP2 dependent ACE2 degradation. Antiviral Res 212:105558 (2023). PubMed: 36806814
  • Li Z  et al. ImmunoPET Imaging Identifies the Optimal Timepoint for Combination Therapy in Xenograft Models of Triple-Negative Breast Cancer. Cancers (Basel) 15:N/A (2023). PubMed: 36900378
  • Sidlik Muskatel R  et al. Correction of T-Cell Repertoire and Autoimmune Diabetes in NOD Mice by Non-myeloablative T-Cell Depleted Allogeneic HSCT. Stem Cells Transl Med 12:281-292 (2023). PubMed: 37184893
  • Inglis AJ  et al. Coupled protein quality control during nonsense-mediated mRNA decay. J Cell Sci 136:N/A (2023). PubMed: 37218462
View all Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Excellent 2nd antibody for WB

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
A excellent 2nd Ab for WB (1:2,000 dilution) used with ab166930 (1:2000).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Baptiste Jude

Verified customer

提交于 Jul 06 2016

excellent antibody for WB assay

Excellent
Abreviews
Abreviews
Application
Western blot
This antibody is good for western blot assay, a dilution of 1 to 5,000 could always offer a good detection signal, which was comparable to the dilution of 1 to 2,000.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 05 2015

good 2nd Ab for WB

Excellent
Abreviews
Abreviews
Application
Western blot
a good 2nd Ab for WB (1:5,000 dilution).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 02 2015

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