驴抗山羊IgG H&L (DyLight® 650)预吸附二抗(ab96938)
Key features and details
- Donkey Anti-Goat IgG H&L (DyLight® 650) preadsorbed
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
概述
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产品名称
驴抗山羊IgG H&L (DyLight® 650)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Goat -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
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经测试应用
适用于: IHC-P, ICC/IF, Flow Cytmore details -
最小交叉反应
Chicken, Horse, Human, Mouse, Pig, Rabbit, Rat more details -
偶联物
DyLight® 650. Ex: 654nm, Em: 673nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
克隆
多克隆 -
同种型
IgG -
常规说明
DyLight® 650 replaces DyLight® 649. -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96938于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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说明 |
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IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (9)
ab96938 被引用在 9 文献中.
- Sydney-Smith JD et al. Delayed viral vector mediated delivery of neurotrophin-3 improves skilled hindlimb function and stability after thoracic contusion. Exp Neurol 360:114278 (2023). PubMed: 36455639
- Crawley-Snowdon H et al. Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29. Nat Commun 11:5031 (2020). PubMed: 33024112
- Kathe C & Moon LDF RNA sequencing dataset describing transcriptional changes in cervical dorsal root ganglia after bilateral pyramidotomy and forelimb intramuscular gene therapy with an adeno-associated viral vector encoding human neurotrophin-3. Data Brief 21:377-385 (2018). PubMed: 30364576
- Scherz B et al. mTh1 driven expression of hTDP-43 results in typical ALS/FTLD neuropathological symptoms. PLoS One 13:e0197674 (2018). PubMed: 29787578
- Rodriguez AM & Downs KM Visceral endoderm and the primitive streak interact to build the fetal-placental interface of the mouse gastrula. Dev Biol N/A:N/A (2017). PubMed: 28882402
- Kullmann FA et al. Urothelial proliferation and regeneration after spinal cord injury. Am J Physiol Renal Physiol 313:F85-F102 (2017). PubMed: 28331065
- Baron L et al. Mycolactone subverts immunity by selectively blocking the Sec61 translocon. J Exp Med 213:2885-2896 (2016). PubMed: 27821549
- Kathe C et al. Intramuscular Neurotrophin-3 normalizes low threshold spinal reflexes, reduces spasms and improves mobility after bilateral corticospinal tract injury in rats. Elife 5:N/A (2016). Rat . PubMed: 27759565
- Rönnlund D et al. Multicolor fluorescence nanoscopy by photobleaching: concept, verification, and its application to resolve selective storage of proteins in platelets. ACS Nano 8:4358-65 (2014). PubMed: 24730587