驴抗山羊IgG H&L (Cy5 ®)预吸附二抗(ab6566)
Key features and details
- Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISA
Related conjugates and formulations
概述
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产品名称
驴抗山羊IgG H&L (Cy5 ®)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Goat -
经测试应用
适用于: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISAmore details -
最小交叉反应
Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details -
免疫原
Goat IgG, whole molecule
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偶联物
Cy5 ®. Ex: 650nm, Em: 667nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis. -
共轭说明
Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG -
克隆
多克隆 -
同种型
IgG -
常规说明
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent dilution.
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| ICC/IF |
1/1000 - 1/5000.
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|
| IHC-P |
Use at an assay dependent concentration.
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|
| IHC-Fr |
Use at an assay dependent concentration.
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| WB | (1) |
Use at an assay dependent dilution.
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| ELISA |
1/10000 - 1/50000.
|
| 说明 |
|---|
|
ICC
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent dilution. |
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ICC/IF
1/1000 - 1/5000. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use at an assay dependent dilution. |
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ELISA
1/10000 - 1/50000. |
图片
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Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)Li Q et al. ADAM17 is critical for multipolar exit and radial migration of neuronal intermediate progenitor cells in mice cerebral cortex. PLoS One 8:e65703 (2013).
Embryonic brains were fixed in 4% paraformaldehyde in PBS overnight and placed in 15% sucrose/PBS for 12 h and then 30% sucrose/PBS for 24 h at 4°C. Brains were then embedded in OCT (optimum cutting temperature) mounting medium and froze before sectioning at 10 or 20 µm. Embryonic cerebral cortex from E14.5, E15.5, E16.5 and E18.5 mice were dissected out and sectioned at 10–15 µm for immunohistochemistry experiment with anti-ADAM17 (ab13535) antibody.
Cy5-labeled donkey anti-goat (ab6566) was used as the secondary antibody (Red).
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Western blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)This image is courtesy of an anonymous AbreviewLanes 2-3 : Goat anti-UCP1 at 1/200 dilution
Lane 1 : Marker
Lanes 2-3 : Mouse brown adipose tissue lysate at 70 µg
Secondary
Lanes 2-3 : Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) at 1/2500 dilution
Observed band size: 33 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (42)
ab6566 被引用在 42 文献中.
- Hennegan J et al. Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development. iScience 27:108670 (2024). PubMed: 38155767
- Li H et al. Alterations of CD8+ T cells in the blood and salivary glands of patients with primary Sjögren's syndrome. Clin Rheumatol 42:1327-1338 (2023). PubMed: 36609932
- Zhao LM et al. Promotion of right ventricular outflow tract reconstruction using a novel cardiac patch incorporated with hypoxia-pretreated urine-derived stem cells. Bioact Mater 14:206-218 (2022). PubMed: 35310356
- Ferreira JV et al. LAMP2A regulates the loading of proteins into exosomes. Sci Adv 8:eabm1140 (2022). PubMed: 35333565
- Yoshida Y et al. Differing impact of phosphoglycerate mutase 1-deficiency on brown and white adipose tissue. iScience 25:104268 (2022). PubMed: 35521515