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驴抗山羊IgG H&L (Cy2 ®)预吸附二抗(ab6948)

Submit a review Q&A (1)References (2)

Key features and details

  • Donkey Anti-Goat IgG H&L (Cy2 ®) preadsorbed
  • Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
  • Host species: Donkey
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm HRP HRP polymer PE Texas Red ® TRITC Unconjugated Unconjugated

概述

  • 产品名称

    驴抗山羊IgG H&L (Cy2 ®)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Donkey

  • 靶标种属

    Goat

  • 经测试应用

    适用于: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details

  • 最小交叉反应


    Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details

  • 免疫原

    Full length native Goat IgG (purified).

  • 偶联物

    Cy2 ®. Ex: 489nm, Em: 505nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

  • 共轭说明

    Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 3.9 moles Cy2 per mole of Donkey IgG

  • 克隆

    多克隆

  • 常规说明

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6948于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.
说明
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (2)

发表研究结果有使用 ab6948？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6948 被引用在 2 文献中.

  • Amo L  et al. Podocalyxin Expressed in Antigen Presenting Cells Promotes Interaction With T Cells and Alters Centrosome Translocation to the Contact Site. Front Immunol 13:835527 (2022). PubMed: 35711462
  • Barsi JC  et al. Mind bomb1 is a ubiquitin ligase essential for mouse embryonic development and Notch signaling. Mech Dev 122:1106-17 (2005). PubMed: 16061358

客户评价及客户问答

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Question

Dear TechTeam,

Our customer has been waiting for ab97114 for 4 months and soonest delivery is indicated as in May 2012. Would it be possible to find alternative products, or maybe the one with label of close kind to Cy2?

Many thanks,

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Answer

Thank you for your enquiry and you interest in our products. I am very sorry for the inconvenience caused.

I am pleased to inform you that we have an alternative product and it is ab6948: Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy2 ®), pre-adsorbed. It is in stock and available.

In future, I would advise you and your customers to use our advanced search engine designed for selecting compatible secondary antibodies: https://www.abcam.com/index.html?pageconfig=productmap&cl=918

You can type the isotype, target species, host species, format, specificity, conjugates and search for the most suitable products.

If you need any further assistance in the future, please do not hesitate to contact me.

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