驴抗山羊IgG H&L (Biotin) (ab6884)
Key features and details
- Donkey Anti-Goat IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
概述
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产品名称
驴抗山羊IgG H&L (Biotin)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Goat -
经测试应用
适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
免疫原
Full length native Goat IgG (purified).
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偶联物
Biotin
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
克隆
多克隆 -
同种型
IgG -
常规说明
Use donkey anti-goat IgG (H&L) biotin to confidently detect primary antibodies raised in goat in your immunohistochemistry, western blot, ELISA or immunoprecipitation. Get dilutions to get the best results in your IHC, WB, IP, or ELISA with donkey anti-goat IgG (H&L) biotin below.Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule
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研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
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| Dot blot |
Use at an assay dependent dilution.
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| ELISA |
1/180000.
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| IHC-P |
Use at an assay dependent dilution.
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|
| IHC-Fr |
Use at an assay dependent dilution.
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| Immunomicroscopy |
Use at an assay dependent dilution.
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| WB |
1/2000 - 1/10000.
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| 说明 |
|---|
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ICC/IF
1/1000 - 1/5000. |
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Dot blot
Use at an assay dependent dilution. |
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ELISA
1/180000. |
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IHC-P
Use at an assay dependent dilution. |
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IHC-Fr
Use at an assay dependent dilution. |
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Immunomicroscopy
Use at an assay dependent dilution. |
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WB
1/2000 - 1/10000. |
图片
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Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Biotin) (ab6884)Image from Dr WZ Wang et al, BMC Mol Biol. 2009 Jul 6;10:69, Fig 2.ab6884 used to stain samples of whole heads of embryonic day 15 (E15) mice by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
Comparison of four staining methods. The heads were flash-frozen directly in isopentane on dry-ice. Once hardened, they were embedded in O.C.T compound. Frozen sections were postfixed in 4% paraformaldehyde in PBS for 20 minutes and quenched in 1.5% hydrogen peroxide for 30 minutes. Sections were then blocked for 2 hours at room temperature with 5% donkey serum in Tris-buffered saline with 0.1% Triton-X100 and then incubated with a goat anti-Nurr1 primary antibody 1/200 in 1% donkey serum in TBS at 4°C overnight. Biotinylated donkey anti-goat (ab6884) 1/500 in 1% donkey serum in TBS was applied for 2 hours at room temperature and reacted with avidin-biotinylated enzyme complex (ABC) using the Vectastain Elite kit and diaminobenzidene (DAB) according to the manufacturer's instructions. Images were taken directly on the PALM Micr
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab6884 被引用在 22 文献中.
- Ninčević V et al. Renoprotective Effect of Liraglutide Is Mediated via the Inhibition of TGF-Beta 1 in an LLC-PK1 Cell Model of Diabetic Nephropathy. Curr Issues Mol Biol 44:1087-1114 (2022). PubMed: 35723295
- Han Q et al. Targeted inhibition of SIRT6 via engineered exosomes impairs tumorigenesis and metastasis in prostate cancer. Theranostics 11:6526-6541 (2021). PubMed: 33995674
- Nguyen L et al. Survival and Motor Phenotypes in FVB C9-500 ALS/FTD BAC Transgenic Mice Reproduced by Multiple Labs. Neuron 108:784-796.e3 (2020). PubMed: 33022226
- Jeong D et al. LRIG1-Mediated Inhibition of EGF Receptor Signaling Regulates Neural Precursor Cell Proliferation in the Neocortex. Cell Rep 33:108257 (2020). PubMed: 33053360
- Binch A et al. Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues. JOR Spine 3:e1098 (2020). PubMed: 33015573