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驴抗山羊IgG H&L (Alkaline Phosphatase)预吸附二抗(ab7126)

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Key features and details

  • Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed
  • Conjugation: Alkaline Phosphatase
  • Host species: Donkey
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

概述

  • 产品名称

    驴抗山羊IgG H&L (Alkaline Phosphatase)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Donkey

  • 靶标种属

    Goat

  • 特异性

    No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.

  • 经测试应用

    适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • 最小交叉反应


    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details

  • 免疫原

    Goat IgG whole molecule

  • 偶联物

    Alkaline Phosphatase

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 50% Glycerol, 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7126于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/200 - 1/1000.
Dot blot
Use at an assay dependent concentration.
ELISA
1/3000 - 1/12000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/500 - 1/2000.
说明
ICC/IF
1/200 - 1/1000.
Dot blot
Use at an assay dependent concentration.
ELISA
1/3000 - 1/12000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/500 - 1/2000.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab7126？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7126 尚未被引用在任何文献中。

客户评价及客户问答

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Question

Could you suggest anti ab9871 secondary antibodies that can be sued in ELISA and ICC/IF?

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Answer

Thank you for contacting us.

As discussed we have many anti goat antibodies that can be used in ELISA and ICC/IF.

Idealy enzyme conjugated antibodies e.g. HRP, Alkaline phosphatase are the best suitable antibodies in ELISA. However these will not work in Immunofluorescence unless further steps in protocol applied.

We have FITC, TRITC or Cy5 conjugated antibodies in catalogue that can work in Immunofluorescence and Fluorescence ELISA.

Another option is choosing Biotin conjugated secondary antibody and then buying strepavidin or streptavidin conjugated HRP (ab7403).

ab5753, ab6740, ab6884, ab7124, ab7131 coul dbe a good option.

I know it sounds complicated however this is only way to combine ELISA and ICC/IF with one secondary antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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