驴抗山羊IgG H&L (Alexa Fluor® 555) (ab150130)
Key features and details
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 555)
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt
Related conjugates and formulations
概述
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产品名称
驴抗山羊IgG H&L (Alexa Fluor® 555)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Goat -
经测试应用
适用于: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details -
免疫原
Full length protein corresponding to Goat IgG.
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偶联物
Alexa Fluor® 555. Ex: 555nm, Em: 565nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
克隆
多克隆 -
同种型
IgG -
常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150130于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF | (1) |
1/200 - 1/1000.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
1/2000.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
1/200 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
1/2000. |
图片
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ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800 (goat anti-mouse IgG) was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150130 Alexa Fluor® 555 donkey anti-goat IgG (H+L) (yellow) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Immunocytochemsitry/Immunfluorescence analysis of mouse brain organotypic slice labelling GFAP primary with Alexa Fluor® 555 Donkey anti-goat IgG H&L (ab150130). Counter stained with the nuclear marker TO-PRO-3. Clear staining shown with 543nm laser.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (54)
ab150130 被引用在 54 文献中.
- Zhou L et al. Convergence of secretory, endosomal, and autophagic routes in trans-Golgi-associated lysosomes. J Cell Biol 222:N/A (2023). PubMed: 36239631
- Pilz RA et al. Endothelial Differentiation of CCM1 Knockout iPSCs Triggers the Establishment of a Specific Gene Expression Signature. Int J Mol Sci 24:N/A (2023). PubMed: 36835400
- Buzova D et al. Profiling of cell-free DNA methylation and histone signatures in pediatric NAFLD: A pilot study. Hepatol Commun 6:3311-3323 (2022). PubMed: 36264206
- Aljobaily N et al. Low-Dose Administration of Cannabigerol Attenuates Inflammation and Fibrosis Associated with Methionine/Choline Deficient Diet-Induced NASH Model via Modulation of Cannabinoid Receptor. Nutrients 15:N/A (2022). PubMed: 36615835
- Kanno S et al. Establishment of a developmental toxicity assay based on human iPSC reporter to detect FGF signal disruption. iScience 25:103770 (2022). PubMed: 35146387